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  5. CD36 Protein, Rat (HEK293, Fc)

CD36 Protein, Rat (HEK293, Fc)

Cat. No.: HY-P74303
Handling Instructions

CD36 is a multifunctional glycoprotein that serves as a receptor for a variety of ligands, including thrombospondin, collagen, and oxidized low-density lipoprotein. Its multivalent ligand induces CD36 clusters, initiating signal transduction. CD36 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD36 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD36 Protein, Rat (HEK293, Fc) is 410 a.a., with molecular weight of ~108 kDa.

For research use only. We do not sell to patients.

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Description

CD36 is a multifunctional glycoprotein that serves as a receptor for a variety of ligands, including thrombospondin, collagen, and oxidized low-density lipoprotein. Its multivalent ligand induces CD36 clusters, initiating signal transduction. CD36 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD36 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of CD36 Protein, Rat (HEK293, Fc) is 410 a.a., with molecular weight of ~108 kDa.

Background

CD36 is a multifunctional glycoprotein serving as a receptor for a diverse array of ligands encompassing proteinaceous entities like thrombospondin, fibronectin, collagen, or amyloid-beta, as well as lipidic components including oxidized low-density lipoprotein (oxLDL), anionic phospholipids, long-chain fatty acids, and bacterial diacylated lipopeptides. Due to their multivalent nature, these ligands can engage multiple receptors concurrently, prompting the formation of CD36 clusters that initiate signal transduction and the internalization of receptor-ligand complexes. The ensuing cellular responses play pivotal roles in angiogenesis, inflammatory responses, fatty acid metabolism, and the processing of taste and dietary fats in the intestine. CD36's involvement in binding long-chain fatty acids facilitates their transport into cells, contributing to muscle lipid utilization, adipose energy storage, and gut fat absorption. Mechanistically, fatty acid binding activates downstream kinase LYN, leading to the phosphorylation and inactivation of palmitoyltransferase ZDHHC5, subsequently resulting in CD36 depalmitoylation and caveolar endocytosis. In the small intestine, CD36 participates in the proximal absorption of dietary fatty acids and cholesterol, potentially through the activation of the MAPK1/3 (ERK1/2) signaling pathway. Furthermore, CD36 is crucial for oral fat perception and preferences, with its presence in taste receptor cells mediating intracellular calcium level increases and activating gustatory neurons. In the ventromedial hypothalamus, CD36 plays a pivotal role in sensing long-chain fatty acids and regulating energy and glucose homeostasis. Acting as a receptor for thrombospondins and as a coreceptor for TLR4:TLR6 heterodimer, CD36 promotes antiangiogenic effects and inflammation in monocytes/macrophages, respectively. Upon ligand binding, CD36 interacts with the TLR4:TLR6 heterodimer, leading to internalization and triggering inflammatory responses via NF-kappa-B-dependent pathways. Additionally, CD36 serves as a selective and nonredundant sensor of microbial diacylated lipopeptides, eliciting NF-kappa-B-dependent cytokine production and subsequent Golgi targeting in a lipid-raft dependent pathway through TLR2:TLR6 heterodimer signaling.

Species

Rat

Source

HEK293

Tag

C-hFc

Accession

Q07969 (G30-K439)

Gene ID
Molecular Construction
N-term
CD36 (G30-K439)
Accession # Q07969
hFc
C-term
Synonyms
Platelet glycoprotein 4; GPIIIB; GPIV; CD36; Fat
Molecular Weight

Approximately 108 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

CD36 Protein, Rat (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD36 Protein, Rat (HEK293, Fc)
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HY-P74303
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