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  4. CD39L4/ENTPD5 Protein, Mouse (sf9, His)

CD39L4/ENTPD5 Protein, Mouse (sf9, His)

Cat. No.: HY-P76317
Handling Instructions

The CD39L4/ENTPD5 protein is located in the lumen of the endoplasmic reticulum and plays a crucial role in glycoprotein processing by hydrolyzing nucleoside diphosphates (including GDP, IDP, and UDP). Its enzymatic activity is critical for the clearance of UDP, an end-product feedback inhibitor of UDP-Glc:glycoprotein glucosyltransferase. CD39L4/ENTPD5 Protein, Mouse (sf9, His) is the recombinant mouse-derived CD39L4/ENTPD5 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of CD39L4/ENTPD5 Protein, Mouse (sf9, His) is 409 a.a., with molecular weight of ~45 KDa.

For research use only. We do not sell to patients.

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Description

The CD39L4/ENTPD5 protein is located in the lumen of the endoplasmic reticulum and plays a crucial role in glycoprotein processing by hydrolyzing nucleoside diphosphates (including GDP, IDP, and UDP). Its enzymatic activity is critical for the clearance of UDP, an end-product feedback inhibitor of UDP-Glc:glycoprotein glucosyltransferase. CD39L4/ENTPD5 Protein, Mouse (sf9, His) is the recombinant mouse-derived CD39L4/ENTPD5 protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of CD39L4/ENTPD5 Protein, Mouse (sf9, His) is 409 a.a., with molecular weight of ~45 KDa.

Background

CD39L4/ENTPD5 protein demonstrates its enzymatic activity by hydrolyzing nucleoside diphosphates, with a notable preference for GDP, IDP, and UDP over ADP and CDP. Particularly, within the endoplasmic reticulum lumen, CD39L4/ENTPD5 plays a vital role in clearing UDP, which acts as an end-product feedback inhibitor of the UDP-Glc:glycoprotein glucosyltransferases. By hydrolyzing UDP, CD39L4/ENTPD5 promotes the regeneration of UDP-glucose in the cytosol through an antiporter mechanism, ensuring a positive regulation of protein reglucosylation. This process is crucial for proper glycoprotein folding and quality control within the endoplasmic reticulum, emphasizing CD39L4/ENTPD5's significance in maintaining cellular functions related to glycoprotein processing.

Species

Mouse

Source

Sf9 insect cells

Tag

C-His

Accession

Q9WUZ9 (T19-S427)

Gene ID
Molecular Construction
N-term
ENTPD5 (T19-S427)
Accession # Q9WUZ9
His
C-term
Synonyms
Ectonucleoside triphosphate diphosphohydrolase 5; NTPDase 5; CD39 antigen-like 4; CD39L4
Molecular Weight

Approximately 45 kDa.

Purity

Greater than 90% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

CD39L4/ENTPD5 Protein, Mouse (sf9, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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CD39L4/ENTPD5 Protein, Mouse (sf9, His)
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HY-P76317
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