CD4 Protein, Mouse (HEK293, His)

CD4 is a glycoprotein critical in immune responses that binds to interleukin-16 and forms a homodimer. It enhances helper T cell-mediated immune responses and regulates processes such as defense against Gram-negative bacteria and calcium signaling. CD4 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CD4 protein, expressed by HEK293 , with C-His, C-6*His labeled tag. The total length of CD4 Protein, Mouse (HEK293, His) is 368 a.a., with molecular weight of 43-60 kDa.

CD4, a key glycoprotein involved in immune responses, is predicted to perform various functions, including interleukin-16 binding activity, interleukin-16 receptor activity, and protein homodimerization activity. Playing a critical role in helper T cell enhancement of the adaptive immune response, CD4 acts upstream of processes such as the defense response to Gram-negative bacteria, positive regulation of calcium-mediated signaling, and positive regulation of peptidyl-tyrosine phosphorylation. Positioned in the endoplasmic reticulum, on the external side of the plasma membrane, and within membrane rafts, CD4 exhibits biased expression in different tissues, particularly evident in the thymus with an expression level of RPKM 167.0, and the spleen with RPKM 15.0, underscoring its significance in immune function and its potential involvement in immunodeficiency 79.

Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. The ED50 this effect is ≤3 μg/mL, corresponding to a specific activity is ≥355.333 units/mg.

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  Measured by the ability of the immobilized protein to support the adhesion of NIH‑3T3 mouse embryonic fibroblast cells. The ED₅₀ this effect is 2.816 μg/mL, corresponding to a specific activity is 355.114 units/mg.

Mouse

HEK293

C-6*His

NP_038516.1 (K27-T394)

12504  [NCBI]

KTLVLGKEGESAELPCESSQKKITVFTWKFSDQRKILGQHGKGVLIRGGSPSQFDRFDSKKGAWEKGSFPLIINKLKMEDSQTYICELENRKEEVELWVFKVTFSPGTSLLQGQSLTLTLDSNSKVSNPLTECKHKKGKVVSGSKVLSMSNLRVQDSDFWNCTVTLDQKKNWFGMTLSVLGFQSTAITAYKSEGESAEFSFPLNFAEENGWGELMWKAEKDSFFQPWISFSIKNKEVSVQKSTKDLKLQLKETLPLTLKIPQVSLQFAGSGNLTLTLDKGTLHQEVNLVVMKVAQLNNTLTCEVMGPTSPKMRLTLKQENQEARVSEEQKVVQVVAPETGLWQCLLSEGDKVKMDSRIQVLSRGVNQT

43-60kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.