CD64 Protein, Mouse (HEK293, His-Avi)

The CD64 protein is a high-affinity receptor for the immunoglobulin gamma Fc region and plays a key role in innate and adaptive immune responses. It prevents FCGR1A degradation by interacting with FCERG1 and FLNA to form a functional signaling complex. CD64 Protein, Mouse (HEK293, His-Avi) is the recombinant mouse-derived CD64 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of CD64 Protein, Mouse (HEK293, His-Avi) is 273 a.a., with molecular weight of 45-50 kDa.

CD64 Protein emerges as a high-affinity receptor specifically designed for the Fc region of immunoglobulins gamma, playing a pivotal role in both innate and adaptive immune responses. Its dynamic functionality extends to forming a functional signaling complex through interaction with FCERG1. Additionally, CD64 interacts with FLNA, preventing FCGR1A degradation, and engages with EPB41L2, LAT, and PPL in intricate cellular processes. Furthermore, this receptor establishes connections with HCK and LYN, showcasing its versatility and involvement in various signaling cascades critical for immune system modulation.

Measured by its binding ability in a functional ELISA. Immobilized CD64 Protein, Mouse (HEK293, His-Avi) at 10 μg/mL (100 μl/well) can bind human IgG1 and the EC₅₀ is 0.07-0.15 μg/mL. Labeled biotin to CD64 Protein, Mouse (HEK293, His-Avi) by a certain molar ratio; Using the Octet RED System, the affinity constant (Kd) of CD64 Protein, Mouse (HEK293, His-Avi) bound to IgG1 Antibody was 5.6 nM.

Mouse

HEK293

C-Avi;C-His

P26151 (E25-P297)

14129  [NCBI]

EVVNATKAVITLQPPWVSIFQKENVTLWCEGPHLPGDSSTQWFINGTAVQISTPSYSIPEASFQDSGEYRCQIGSSMPSDPVQLQIHNDWLLLQASRRVLTEGEPLALRCHGWKNKLVYNVVFYRNGKSFQFSSDSEVAILKTNLSHSGIYHCSGTGRHRYTSAGVSITVKELFTTPVLRASVSSPFPEGSLVTLNCETNLLLQRPGLQLHFSFYVGSKILEYRNTSSEYHIARAEREDAGFYWCEVATEDSSVLKRSPELELQVLGPQSSAP

45-50 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.