CD98 Protein, Rat (HEK293, Fc)

CD98 Protein, a chaperone, aids in the biogenesis and trafficking of functional transporter heterodimers to the plasma membrane. It forms heterodimers with SLC7 family transporters, acting as amino acid antiporters with substrate specificity determined by the SLC7A subunit. CD98 is crucial for plasma membrane localization, stability, and transport activity of SLC7A5 and SLC7A8. When associated with LAPTM4B, the SLC7A5 heterodimer is recruited to lysosomes, promoting leucine uptake and mTORC1 activation. CD98 modulates integrin-related signaling and is essential for integrin-dependent cell spreading, migration, and tumor progression. Additionally, it interacts with TLCD3A/CT120, ICAM1, and beta-1 integrins. CD98 Protein, Rat (HEK293, Fc) is the recombinant rat-derived CD98 protein, expressed by HEK293, with N-hFc labeled tag. The total length of CD98 Protein, Rat (HEK293, Fc) is 428 a.a., with molecular weight of ~95 kDa.

CD98 protein functions as a chaperone, facilitating the biogenesis and trafficking of functional transporter heterodimers to the plasma membrane. It forms heterodimers with various SLC7 family transporters, including SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10, and SLC7A11, acting as amino acid antiporters with substrate specificity determined by the SLC7A subunit. For example, heterodimers formed by SLC3A2/SLC7A6 or SLC3A2/SLC7A7 mediate the uptake of dibasic amino acids, while the SLC3A2/SLC7A11 heterodimer functions as an antiporter by exchanging extracellular anionic L-cystine and intracellular L-glutamate across the cellular plasma membrane. CD98 is essential for the plasma membrane localization, stability, and transport activity of SLC7A5 and SLC7A8. When associated with LAPTM4B, the SLC7A5 heterodimer is recruited to lysosomes, promoting leucine uptake and mTORC1 activation. CD98 also modulates integrin-related signaling and is crucial for integrin-dependent cell spreading, migration, and tumor progression. It forms a disulfide-linked heterodimer with a non-glycosylated light chain (SLC7A5, SLC7A6, SLC7A7, SLC7A8, SLC7A10, or SLC7A11) and interacts with TLCD3A/CT120 and ICAM1. Additionally, CD98 constitutively and specifically associates with beta-1 integrins, including alpha-2/beta-1, alpha-3/beta-1, alpha-5/beta-1, and alpha-6/beta-1, with minimal interaction with alpha-4/beta-1. It also interacts with LAPTM4B, recruiting SLC3A2 and SLC7A5 to lysosomes for leucine uptake and mTORC1 activation.

Rat

HEK293

N-hFc

Q794F9 (A100-A527)

50567  [NCBI]

APRCRELPVQRWWHKGALYRIGDLQAFVGPEARGIAGLKNHLEYLSTLKVKGLVLGPIHKNQKDEVNETDLKQIDPDLGSQEDFKDLLQSAKKKSIHIILDLTPNYKGQNAWFLPPQADIVATKMKEALSSWLQDGVDGFQVRDVGKLANASLYLAEWQNITKNFSEDRLLIAGTASSDLQQIVNILESTSDLLLTSSYLSQPVFTGEHAELLVIKYLNATGSRWCSWSVSQAGLLTSFIPAQFLRLYQLLLFTLPGTPVFSYGDELGLQAVALPGQPMEAPFMLWNESSNSQTSSPVSLNMTVKGQNEDPGSLLTQFRRLSDLRGKERSLLHGDFDALSSSSGLFSYVRHWDQNERYLVVLNFQDVGLSARVGASNLPAGISLPASANLLLSTDSTRLSREEGTSLSLENLSLNPYEGLLLQFPFVA

Approximately 95 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in PBS, pH 7.4. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.