CDK3-CCNE1 Heterodimer Protein, Human (Sf9, GST, FLAG, His)

CDK3 protein is a serine/threonine protein kinase that plays a key role in regulating the eukaryotic cell cycle, especially affecting the G0-G1 and G1-S transitions. It interacts with CCNC/cyclin-C during interphase and phosphorylates substrates such as histone H1, ATF1, RB1, and CABLES1. CDK3-CCNE1 Heterodimer Protein, Human (Sf9, GST, FLAG, His) is a recombinant protein dimer complex containing human-derived CDK3-CCNE1 Heterodimer protein, expressed by Sf9 insect cells , with N-6*His, N-Flag, N-GST labeled tag.

CDK3 Protein is a serine/threonine-protein kinase that assumes a pivotal role in the regulation of the eukaryotic cell cycle, specifically influencing G0-G1 and G1-S transitions. During interphase, it interacts with CCNC/cyclin-C. The kinase phosphorylates key substrates, including histone H1, ATF1, RB1, and CABLES1. ATF1 phosphorylation induced by CDK3-CCNE1 triggers ATF1 transactivation and transcriptional activities, consequently fostering cell proliferation and transformation. Moreover, the CDK3/cyclin-C-mediated phosphorylation of RB1 is essential for the G0-G1 transition. Notably, CDK3-CCNE1 contributes to the G1-S transition, potentially by participating in the activation of E2F1, E2F2, and E2F3 in a RB1-independent manner. This intricate interplay positions CDK3-CCNE1 as a crucial orchestrator of cell cycle progression.

Human

Sf9 insect cells

N-6*His;N-Flag;N-GST

Q00526 (M1-H305)&P24864 (M1-E410)

1018&898

61.6 kDa&41.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.