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Cerberus 1/CER1 Protein, Human (HEK293, His)

Cat. No.: HY-P7822
COA Handling Instructions

The CER1 protein, a DAN family BMP antagonist, coordinates growth-promoting ureteric bud signaling by inducing BMP inhibition. CER1 also regulates embryogenesis and carcinogenesis by inhibiting NODAL signaling to SMAD2 or SMAD3 through ALK4/ALK7. Cerberus 1/CER1 Protein, Human (HEK293, His) is the recombinant human-derived Cerberus 1/CER1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cerberus 1/CER1 Protein, Human (HEK293, His) is 250 a.a., with molecular weight of 35-42 kDa.

For research use only. We do not sell to patients.

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10 μg $140 In-stock
50 μg $380 In-stock
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Description

The CER1 protein, a DAN family BMP antagonist, coordinates growth-promoting ureteric bud signaling by inducing BMP inhibition. CER1 also regulates embryogenesis and carcinogenesis by inhibiting NODAL signaling to SMAD2 or SMAD3 through ALK4/ALK7. Cerberus 1/CER1 Protein, Human (HEK293, His) is the recombinant human-derived Cerberus 1/CER1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cerberus 1/CER1 Protein, Human (HEK293, His) is 250 a.a., with molecular weight of 35-42 kDa.

Background

Cerberus 1, DAN family BMP antagonist (CER1), is a cysteine knot superfamily protein homolog and a DAN family BMP antagonist. CER1 has nine conserved cysteines and a cysteine knot region, together with Dan and DRM/Gremlin represent bone morphogenetic protein (BMP) antagonists that can directly bind to BMP and inhibit its activity. Upregulation of CER1 in human ES cells leads to inhibition of Nodal signaling associated with human ES cell differentiation. CER1 (Cerberus 1) and GREM3 (CKTSF1B3 or CER2) inhibit NODAL signaling through ACVR1B (ALK4) or ACVR1C (ALK7) to SMAD2 or SMAD3. The network formed by the Nodal and BMP signaling pathways and the WNT signaling pathway is related to embryogenesis and carcinogenesis. Although, CER1 inhibits Wnt3a-induced classical Top Flash reporter to some extent, it does not affect Wnt11 signaling in cell culture. Furthermore, CER1 fine-tunes the spatial organization of the ureteral tree by coordinating the activities of the growth-promoting ureteric bud signals Gndf and Wnt11 through Bmp-mediated antagonism and, to some extent, canonical Wnt signaling involved in branching.

Species

Human

Source

HEK293

Tag

C-6*His

Accession

AAI03977.1 (T18-A267)

Gene ID
Molecular Construction
N-term
CER1 (T18-A267)
Accession # AAI03977.1
6*His
C-term
Synonyms
rHuCerberus 1, cysteine knot superfamily, homolog (Xenopus laevis)/CER1, His; Cerberus; Cerberus-Related Protein; DAN Domain Family Member 4; CER1; DAND4
AA Sequence

TRHQDGRQNQSSLSPVLLPRNQRELPTGNHEEAEEKPDLFVAVPHLVGTSPAGEGQRQREKMLSRFGRFWKKPEREMHPSRDSDSEPFPPGTQSLIQPIDGMKMEKSPLREEAKKFWHHFMFRKTPASQGVILPIKSHEVHWETCRTVPFSQTITHEGCEKLVVQNNLCFGKCGSVHFPGAAQHSHTSCSHCLPAKFTTMHLPLNCTELSSVIKVVMLVEECQCKVKTEHEDGHILHAGSQDSFIPGVSA

Molecular Weight

35-42 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM NaAc-HAC, pH 4.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Cerberus 1/CER1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Cerberus 1/CER1 Protein, Human (HEK293, His)
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HY-P7822
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