Cerberus 1/CER1 Protein, Human (HEK293, His)

The CER1 protein, a DAN family BMP antagonist, coordinates growth-promoting ureteric bud signaling by inducing BMP inhibition. CER1 also regulates embryogenesis and carcinogenesis by inhibiting NODAL signaling to SMAD2 or SMAD3 through ALK4/ALK7. Cerberus 1/CER1 Protein, Human (HEK293, His) is the recombinant human-derived Cerberus 1/CER1 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of Cerberus 1/CER1 Protein, Human (HEK293, His) is 250 a.a., with molecular weight of 35-42 kDa.

Cerberus 1, DAN family BMP antagonist (CER1), is a cysteine knot superfamily protein homolog and a DAN family BMP antagonist. CER1 has nine conserved cysteines and a cysteine knot region, together with Dan and DRM/Gremlin represent bone morphogenetic protein (BMP) antagonists that can directly bind to BMP and inhibit its activity. Upregulation of CER1 in human ES cells leads to inhibition of Nodal signaling associated with human ES cell differentiation. CER1 (Cerberus 1) and GREM3 (CKTSF1B3 or CER2) inhibit NODAL signaling through ACVR1B (ALK4) or ACVR1C (ALK7) to SMAD2 or SMAD3. The network formed by the Nodal and BMP signaling pathways and the WNT signaling pathway is related to embryogenesis and carcinogenesis. Although, CER1 inhibits Wnt3a-induced classical Top Flash reporter to some extent, it does not affect Wnt11 signaling in cell culture. Furthermore, CER1 fine-tunes the spatial organization of the ureteral tree by coordinating the activities of the growth-promoting ureteric bud signals Gndf and Wnt11 through Bmp-mediated antagonism and, to some extent, canonical Wnt signaling involved in branching.

Human

HEK293

C-6*His

AAI03977.1 (T18-A267)

9350  [NCBI]

TRHQDGRQNQSSLSPVLLPRNQRELPTGNHEEAEEKPDLFVAVPHLVGTSPAGEGQRQREKMLSRFGRFWKKPEREMHPSRDSDSEPFPPGTQSLIQPIDGMKMEKSPLREEAKKFWHHFMFRKTPASQGVILPIKSHEVHWETCRTVPFSQTITHEGCEKLVVQNNLCFGKCGSVHFPGAAQHSHTSCSHCLPAKFTTMHLPLNCTELSSVIKVVMLVEECQCKVKTEHEDGHILHAGSQDSFIPGVSA

35-42 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM NaAc-HAC, pH 4.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.