cGAS Protein, Human (His)

The cGAS protein is a nucleotidyl transferase that crucially mediates innate immunity by catalyzing the formation of 2',3'-cGAMP from ATP and GTP upon binding to double-stranded DNA (dsDNA). As a DNA sensor, cGAS detects exogenous and endogenous DNA, triggering STING1 activation and type I interferon production. cGAS Protein, Human (His) is the recombinant human-derived cGAS protein, expressed by E. coli , with C-6*His labeled tag. The total length of cGAS Protein, Human (His) is 522 a.a., with molecular weight of ~58.9 kDa.

cGAS Protein, a nucleotidyltransferase, plays a pivotal role in innate immunity by catalyzing the formation of cyclic GMP-AMP (2',3'-cGAMP) from ATP and GTP. Acting as a key DNA sensor, cGAS directly binds double-stranded DNA (dsDNA), inducing the synthesis of 2',3'-cGAMP, a second messenger that activates STING1, leading to the production of type-I interferon and triggering immune responses. Recognizing curved long dsDNAs, cGAS acts as a foreign DNA sensor, detecting the presence of DNA from viruses, retroviruses, bacteria, and neutrophil extracellular traps (NETs). Moreover, cGAS responds to endogenous DNA released during cellular stress, contributing to sterile inflammation. It regulates cellular senescence by binding to cytosolic chromatin fragments and participates in the inflammatory response to genome instability and double-stranded DNA breaks. cGAS is also implicated in sensing translation stress, and its activation inhibits homologous recombination repair in response to DNA damage. Human cGAS exhibits species-specific mechanisms of DNA recognition, allowing a more finely tuned response to pathogens.

Enzyme activity has been tested in vitro.

Human

E. coli

C-6*His

Q8N884-1 (M1-F522)

115004  [NCBI]

MQPWHGKAMQRASEAGATAPKASARNARGAPMDPTESPAAPEAALPKAGKFGPARKSGSRQKKSAPDTQERPPVRATGARAKKAPQRAQDTQPSDATSAPGAEGLEPPAAREPALSRAGSCRQRGARCSTKPRPPPGPWDVPSPGLPVSAPILVRRDAAPGASKLRAVLEKLKLSRDDISTAAGMVKGVVDHLLLRLKCDSAFRGVGLLNTGSYYEHVKISAPNEFDVMFKLEVPRIQLEEYSNTRAYYFVKFKRNPKENPLSQFLEGEILSASKMLSKFRKIIKEEINDIKDTDVIMKRKRGGSPAVTLLISEKISVDITLALESKSSWPASTQEGLRIQNWLSAKVRKQLRLKPFYLVPKHAKEGNGFQEETWRLSFSHIEKEILNNHGKSKTCCENKEEKCCRKDCLKLMKYLLEQLKERFKDKKHLDKFSSYHVKTAFFHVCTQNPQDSQWDRKDLGLCFDNCVTYFLQCLRTEKLENYFIPEFNLFSSNLIDKRSKEFLTKQIEYERNNEFPVFDEF

Approximately 58.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris HCl, 100 mM NaCl, 1 mM DTT, 5% Glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.