Chk1 Protein, Mouse (sf9, His-GST)

Chk1 is a serine/threonine protein kinase that critically coordinates checkpoint-mediated cell cycle arrest and DNA repair in response to DNA damage or unreplicated DNA. It negatively regulates unperturbed cell cycle progression and protects genome integrity by phosphorylating key targets such as CDC25A, CDC25B, CDC25C, RAD51, TP53, histone H3.1, RB1, SPRTN, TLK1 and FANCE. Chk1 Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived Chk1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of Chk1 Protein, Mouse (sf9, His-GST) is 476 a.a., with molecular weight of ~78 kDa.

Chk1, a serine/threonine-protein kinase, plays a crucial role in orchestrating checkpoint-mediated cell cycle arrest and facilitating DNA repair in response to DNA damage or unreplicated DNA. Additionally, it exerts negative regulation on cell cycle progression during unperturbed cell cycles, employing various mechanisms to safeguard genome integrity. Chk1 recognizes the substrate consensus sequence [R-X-X-S/T] and phosphorylates key targets such as CDC25A, CDC25B, CDC25C, and RAD51. Notably, phosphorylation of CDC25A and CDC25C creates binding sites for 14-3-3 proteins, inhibiting their activity and impeding cell cycle progression. Chk1's intricate involvement extends to the phosphorylation of TP53, promoting its activation, leading to cell cycle arrest, and suppressing cellular proliferation. Furthermore, Chk1 phosphorylates histone H3.1, influencing epigenetic regulation of gene expression. Its multifaceted role encompasses the phosphorylation of RB1, SPRTN, TLK1, and FANCE, contributing to the regulation of transcription, chromatin assembly, and DNA repair processes. Chk1 emerges as a pivotal player in maintaining genomic stability and orchestrating cellular responses to diverse challenges.

Mouse

Sf9 insect cells

N-His;N-GST

O35280 (M1-T476)

12649  [NCBI]

Approximately 78 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.