CHRDL2 Protein, Mouse (HEK293, His)

CHRDL2 protein is related to tumor angiogenesis and may negatively regulate BMP activity by hindering the interaction between BMP and receptors. It significantly affects cartilage formation and regeneration, suggesting a role in regulating matrix accumulation of immature mesenchymal cells. CHRDL2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived CHRDL2 protein, expressed by HEK293 , with C-6*His labeled tag.

CHRDL2 Protein is implicated in tumor angiogenesis, potentially acting as a negative regulator of BMPs activity by obstructing their interaction with respective receptors. It exhibits a significant impact on cartilage formation and regeneration, suggesting a role in modulating the rate of matrix accumulation from immature mesenchymal cells. Furthermore, CHRDL2 may play a regulatory role during myoblast and osteoblast differentiation and maturation processes. Notably, CHRDL2 interacts with various members of the BMP family, including GDF5, INHBA, BMP2, BMP4, BMP5, BMP6, and BMP7, indicating its involvement in diverse cellular and developmental pathways.

Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The IC₅₀for this effect is 51.19 ng/mL in the presence of 150 ng/mL of rhBMP-4.

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  Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The IC₅₀for this effect is 51.19 ng/mL in the presence of 150 ng/mL of rhBMP-4.

Mouse

HEK293

C-6*His

Q8VEA6-1 (Q24-L426)

69121  [NCBI]

QARSRSGKVCLFGEKIYTPGQSWHPYLEPQGTIYCVRCTCSENGHVNCYRLRCPPLHCSQPVMEPQQCCPRCVDPHVPSGLRVPLKSCQLNETTYQHGEIFSAQELFPARLSNQCVLCSCIEGHTYCGLMTCPEPSCPTTLPLPDSCCQTCKDRTTESSTEENLTQLQHGERHSQDPCSERRGPSTPAPTSLSSPLGFIPRHFQSVGMGSTTIKIILKEKHKKACTHNGKTYSHGEVWHPTVLSFGPMPCILCTCIDGYQDCHRVTCPTQYPCSQPKKVAGKCCKICPEDEAEDDHSEVISTRCPKVPGQFHVYTLASPSPDSLHRFVLEHEASDQVEMYIWKLVKGIYHLVQIKRVRKQDFQKEAQNFRLLTGTHEGYWTVFLAQTPELKVTASPDKVTKTL

57-73 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.