1. Recombinant Proteins
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  3. Transferases (EC 2)
  4. CSNK2A1 Protein, Human (sf9, GST)

CK2α/CSNK2A1 protein can phosphorylate serine or threonine. It is involved in the regulation of cell cycle, apoptosis, transcription and viral infection, and has protective effects on the spindle assembly checkpoint and p53/TP53-mediated apoptosis. CK2α/CSNK2A1 can also participate in signal transduction by phosphorylating various factors, affecting circadian clock function and virus life cycle. CSNK2A1 Protein, Human (sf9, GST) is the recombinant human-derived CK2 alpha/CSNK2A1 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CK2α/CSNK2A1 protein can phosphorylate serine or threonine. It is involved in the regulation of cell cycle, apoptosis, transcription and viral infection, and has protective effects on the spindle assembly checkpoint and p53/TP53-mediated apoptosis. CK2α/CSNK2A1 can also participate in signal transduction by phosphorylating various factors, affecting circadian clock function and virus life cycle. CSNK2A1 Protein, Human (sf9, GST) is the recombinant human-derived CK2 alpha/CSNK2A1 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

Biological Activity

The specific activity was determined to be 38 nmol/min/mg using casein as substrate.

Species

Human

Source

Sf9 insect cells

Tag

N-GST

Accession

P68400-1 (M1-Q391)

Gene ID
Molecular Construction
N-term
GST
CSNK2A1 (M1-Q391)
Accession # P68400
C-term
Synonyms
Casein kinase II subunit alpha; CK II alpha; CSNK2A1; CK2A1
Molecular Weight

Approximately 65 kDa.

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM GSH, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

CSNK2A1 Protein, Human (sf9, GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
CSNK2A1 Protein, Human (sf9, GST)
Cat. No.:
HY-P76263
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