CSK Protein, Mouse (sf9, His-GST)

CSK protein is a non-receptor tyrosine protein kinase that regulates cell growth, differentiation, migration and immune responses by phosphorylating Src family kinases (SFK) (such as LCK, SRC, HCK, FYN, LYN, CSK or YES1) . This phosphorylation induces SFK to form an inactive conformation, thereby inhibiting signaling by receptors such as T cell receptor (TCR) and B cell receptor (BCR). CSK Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived CSK protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of CSK Protein, Mouse (sf9, His-GST) is 450 a.a., with molecular weight of ~65 kDa.

CSK Protein, a non-receptor tyrosine-protein kinase, holds a pivotal role in the intricate regulation of cell growth, differentiation, migration, and immune response. It accomplishes this by phosphorylating tyrosine residues located in the C-terminal tails of Src-family kinases (SFKs) such as LCK, SRC, HCK, FYN, LYN, CSK, or YES1. This phosphorylation event induces intramolecular interactions between the phosphotyrosine tail and the SH2 domain of SFKs, resulting in an inactive conformation. To inhibit SFKs, CSK is recruited to the plasma membrane through binding to transmembrane proteins or adapter proteins in close proximity. CSK plays a crucial role in suppressing signaling by various surface receptors, including the T-cell receptor (TCR) and B-cell receptor (BCR), by phosphorylating and maintaining several positive effectors such as FYN or LCK in an inactive state, thereby modulating key cellular responses.

Mouse

Sf9 insect cells

N-His;N-GST

P41241 (M1-L450)

12988  [NCBI]

MSAIQAAWPSGTECIAKYNFHGTAEQDLPFCKGDVLTIVAVTKDPNWYKAKNKVGREGIIPANYVQKREGVKAGTKLSLMPWFHGKITREQAERLLYPPETGLFLVRESTNYPGDYTLCVSCEGKVEHYRIMYHASKLSIDEEVYFENLMQLVEHYTTDADGLCTRLIKPKVMEGTVAAQDEFYRSGWALNMKELKLLQTIGKGEFGDVMLGDYRGNKVAVKCIKNDATAQAFLAEASVMTQLRHSNLVQLLGVIVEEKGGLYIVTEYMAKGSLVDYLRSRGRSVLGGDCLLKFSLDVCEAMEYLEGNNFVHRDLAARNVLVSEDNVAKVSDFGLTKEASSTQDTGKLPVKWTAPEALREKKFSTKSDVWSFGILLWEIYSFGRVPYPRIPLKDVVPRVEKGYKMDAPDGCPPAVYEVMKNCWHLDAATRPTFLQLREQLEHIKTHELHL

Approximately 65 kDa

Greater than 85% as determined by reducing SDS-PAGE

<1 EU/μg, determined by LAL method.