CSNK2A2 Protein, Human (His-SUMO)

CSNK2A2 is the catalytic subunit of the serine/threonine protein kinase complex. It regulates important processes such as cell cycle, apoptosis, and transcription, and affects viral infection. During mitosis, CSNK2A2 maintains CDK1 activity and induces cell cycle arrest. In addition, CSNK2A2 phosphorylates p53/TP53 and negatively regulates apoptosis. It widely affects cell signaling, phosphorylated caspases, the apoptotic factor NOL3, and RNA polymerase, among others. CSNK2A2 also regulates Wnt signaling and acts as a kinase for extracellular proteins. CSNK2A2 Protein, Human (His-SUMO) is the recombinant human-derived CSNK2A2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of CSNK2A2 Protein, Human (His-SUMO) is 343 a.a., with molecular weight of 56.7 kDa.

The catalytic subunit of CSNK2A2 protein functions as a core component of a constitutively active serine/threonine-protein kinase complex, showcasing a broad substrate specificity for acidic residues C-terminal to the phosphorylated serine or threonine. Operating as a regulatory node, CSNK2A2 plays a pivotal role in diverse cellular processes, including cell cycle progression, apoptosis, transcription, and viral infection. It contributes to the p53/TP53-dependent spindle assembly checkpoint during mitosis, maintaining cyclin-B-CDK1 activity and G2 arrest in response to spindle damage. Additionally, CSNK2A2 is implicated in apoptotic regulation, phosphorylating key players like caspases and NOL3. Its influence extends to transcriptional regulation, with direct phosphorylation of RNA polymerases and numerous transcription factors. In the intricate landscape of cellular signaling, CSNK2A2 participates in Wnt signaling, acts as an ectokinase for extracellular proteins, and phosphorylates various proteins involved in the viral life cycles of diverse viruses. Moreover, it plays a crucial role in the circadian clock function and contributes to the formation of a membraneless compartment by phosphorylating FMR1. Overall, CSNK2A2 emerges as a central orchestrator of cellular responses to diverse stimuli, showcasing its significance in maintaining cellular homeostasis.

Human

E. coli

N-SUMO;N-6*His

P68400 (R8-N350)

1457  [NCBI]

RARVYTDVNTHRPREYWDYESHVVEWGNQDDYQLVRKLGRGKYSEVFEAINITNNEKVVVKILKPVKKKKIKREIKILENLRGGPNIITLADIVKDPVSRTPALVFEHVNNTDFKQLYQTLTDYDIRFYMYEILKALDYCHSMGIMHRDVKPHNVMIDHEHRKLRLIDWGLAEFYHPGQEYNVRVASRYFKGPELLVDYQMYDYSLDMWSLGCMLASMIFRKEPFFHGHDNYDQLVRIAKVLGTEDLYDYIDKYNIELDPRFNDILGRHSRKRWERFVHSENQHLVSPEALDFLDKLLRYDHQSRLTAREAMEHPYFYTVVKDQARMGSSSMPGGSTPVSSAN

56.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.