D-amino-acid oxidase/DAO Protein, Human (His)

D-amino-acid oxidase Protein, Human (His) is a recombinant D-amino-acid oxidase protein with a His tag. D-amino acid oxidase (DAO) is a flavin adenine dinucleotide (FAD)-dependent oxidase metabolizing neutral and polar D-amino acids^[1].

D-amino acid oxidase (DAO) is a flavin adenine dinucleotide (FAD)–dependent peroxisomal enzyme that catalyzes the oxidation of neutral and polar D-amino acids with a strict stereospecificity to give α-keto acids, ammonia, and reactive oxygen species (ROS)^[1].

Measured by its ability to produce hydrogen peroxide during the oxidative deamination of D-amino acids that incubate the reactions for 10 minutes at 37 °C. The specific activity is 4565.417 pmol/min/μg.

Human

E. coli

N-6*His

P14920 (M1-L347)

1610  [NCBI]

MRVVVIGAGVIGLSTALCIHERYHSVLQPLDIKVYADRFTPLTTTDVAAGLWQPYLSDPNNPQEADWSQQTFDYLLSHVHSPNAENLGLFLISGYNLFHEAIPDPSWKDTVLGFRKLTPRELDMFPDYGYGWFHTSLILEGKNYLQWLTERLTERGVKFFQRKVESFEEVAREGADVIVNCTGVWAGALQRDPLLQPGRGQIMKVDAPWMKHFILTHDPERGIYNSPYIIPGTQTVTLGGIFQLGNWSELNNIQDHNTIWEGCCRLEPTLKNARIIGERTGFRPVRPQIRLEREQLRTGPSNTEVIHNYGHGGYGLTIHWGCALEAAKLFGRILEEKKLSRMPPSHL

Approximately 40.0 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 20% Glycerol, 1 mM DTT, 2 mM EDTA, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. Nagano T, et al. d-amino acid oxidase promotes cellular senescence via the production of reactive oxygen species. Life Sci Alliance. 2019;2(1):e201800045. Published 2019 Jan 18.  [Content Brief]