Dipeptidyl peptidase 3/DPP3 Protein, Human (His)

According to research reports, dipeptidyl peptidase 3 (DPP3) protein, as an enzymatic entity, is able to cleave and degrade various bioactive peptides, including substrates such as angiotensin, leucine-enkephalin, and methionine-enkephalin. According to the results of multiple studies, in addition to its role in processing these bioactive peptides, DPP3 also has the ability to cleave Arg-Arg-β-naphthylamide in vitro. Dipeptidyl peptidase 3/DPP3 Protein, Human (His) is the recombinant human-derived Dipeptidyl peptidase 3/DPP3 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Dipeptidyl peptidase 3/DPP3 Protein, Human (His) is 737 a.a., with molecular weight of 76-88 kDa.

Dipeptidyl peptidase 3 (DPP3) is a protein with significant roles in peptide metabolism, cleaving and degrading various bioactive peptides, including angiotensin, Leu-enkephalin, and Met-enkephalin. This enzymatic activity suggests its involvement in the regulation of key physiological processes, particularly those related to blood pressure and pain modulation. Additionally, DPP3 has been shown to cleave Arg-Arg-beta-naphthylamide in vitro, further expanding its substrate specificity. The diverse range of peptides targeted by DPP3 underscores its importance in modulating the levels of signaling molecules and highlights its potential implications in various physiological and pathological contexts (

Measured by its ability to cleave the fluorogenic peptide substrate, Arg-Arg-7-amido-4-methylcoumarin (RR-AMC, HY-P4340) that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 2417.68 pmol/min/μg.

Human

E. coli

N-6*His

Q9NY33 (M1-A737)

10072  [NCBI]

MADTQYILPNDIGVSSLDCREAFRLLSPTERLYAYHLSRAAWYGGLAVLLQTSPEAPYIYALLSRLFRAQDPDQLRQHALAEGLTEEEYQAFLVYAAGVYSNMGNYKSFGDTKFVPNLPKEKLERVILGSEAAQQHPEEVRGLWQTCGELMFSLEPRLRHLGLGKEGITTYFSGNCTMEDAKLAQDFLDSQNLSAYNTRLFKEVDGEGKPYYEVRLASVLGSEPSLDSEVTSKLKSYEFRGSPFQVTRGDYAPILQKVVEQLEKAKAYAANSHQGQMLAQYIESFTQGSIEAHKRGSRFWIQDKGPIVESYIGFIESYRDPFGSRGEFEGFVAVVNKAMSAKFERLVASAEQLLKELPWPPTFEKDKFLTPDFTSLDVLTFAGSGIPAGINIPNYDDLRQTEGFKNVSLGNVLAVAYATQREKLTFLEEDDKDLYILWKGPSFDVQVGLHELLGHGSGKLFVQDEKGAFNFDQETVINPETGEQIQSWYRSGETWDSKFSTIASSYEECRAESVGLYLCLHPQVLEIFGFEGADAEDVIYVNWLNMVRAGLLALEFYTPEAFNWRQAHMQARFVILRVLLEAGEGLVTITPTTGSDGRPDARVRLDRSKIRSVGKPALERFLRRLQVLKSTGDVAGGRALYEGYATVTDAPPECFLTLRDTVLLRKESRKLIVQPNTRLEGSDVQLLEYEASAAGLIRSFSERFPEDGPELEEILTQLATADARFWKGPSEAPSGQA

76-88 kDa

Greater than 95% as determined by reducing SDS-PAGE

Solution.

Supplied as a 0.2 μm filtered solution of 25 mM Tris-HCl, 10 mM NaCl, 2 mM DTT, 0.05% Tween 80, 20% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.