DKK-3 Protein, Human (HEK293, His)

DKK-3 protein antagonizes canonical Wnt signaling by blocking LRP5/6-Wnt interactions and forming a ternary complex with KREMEN, thereby promoting LRP5/6 internalization.In vertebrate development, DKK-3, together with other DKKs, critically inhibits Wnt-regulated processes such as axial patterning, limb development, somitogenesis, and eye formation.DKK-3 Protein, Human (HEK293, His) is the recombinant human-derived DKK-3 protein, expressed by HEK293 , with C-6*His labeled tag.

The DKK-3 protein functions as an antagonist of canonical Wnt signaling by impeding the interaction between LRP5/6 and Wnt and forming a ternary complex with the transmembrane protein KREMEN, facilitating the internalization of LRP5/6. Within vertebrate development, DKKs, including DKK-3, play a crucial role by locally inhibiting Wnt-regulated processes such as antero-posterior axial patterning, limb development, somitogenesis, and eye formation. In the adult, DKKs are implicated in diverse physiological and pathological conditions, encompassing bone formation, bone disease, cancer, and Alzheimer's disease (By similarity). Notably, DKK-3's interaction with LRP5 and LRP6 highlights its regulatory involvement in inhibiting Wnt signaling and its potential impact on various cellular processes.

Human

HEK293

C-6*His

Q9UBP4 (A22-I350)

27122  [NCBI]

APAPTATSAPVKPGPALSYPQEEATLNEMFREVEELMEDTQHKLRSAVEEMEAEEAAAKASSEVNLANLPPSYHNETNTDTKVGNNTIHVHREIHKITNNQTGQMVFSETVITSVGDEEGRRSHECIIDEDCGPSMYCQFASFQYTCQPCRGQRMLCTRDSECCGDQLCVWGHCTKMATRGSNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPASRLLDLITWELEPDGALDRCPCASGLLCQPHSHSLVYVCKPTFVGSRDQDGEILLPREVPDEYEVGSFMEEVRQELEDLERSLTEEMALGEPAAAAAALLGGEEI

Approximately 66-73 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.