DKK-3 Protein, Mouse (HEK293, His)

DKK-3 protein antagonizes canonical Wnt signaling by inhibiting LRP5/6-Wnt interaction. It forms a ternary complex with KREMEN and promotes LRP5/6 internalization. DKK-3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived DKK-3 protein, expressed by HEK293 , with C-His labeled tag.

The DKK-3 protein is involved in antagonizing canonical Wnt signaling by inhibiting the interaction between LRP5/6 and Wnt. It achieves this through the formation of a ternary complex with the transmembrane protein KREMEN, which facilitates the internalization of LRP5/6. DKKs, including DKK-3, are crucial in vertebrate development as they locally inhibit Wnt-regulated processes such as antero-posterior axial patterning, limb development, somitogenesis, and eye formation. In adult organisms, DKKs are implicated in bone formation, bone diseases, cancer, and Alzheimer's disease. Additionally, DKK-3 interacts with LRP5 and LRP6 proteins. It is important to note that DKK-3 does not possess carboxypeptidase activity or dipeptidyl-peptidase IV type activity.

Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED₅₀ for this effect is 4.628 μg/mL, corresponding to a specific activity is 216.076 units/mg.

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  Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED₅₀ for this effect is 4.628 μg/mL, corresponding to a specific activity is 216.076 units/mg.

Mouse

HEK293

C-6*His

Q9QUN9 (P23-I349)

50781  [NCBI]

PSPTVTWTPAEPGPALNYPQEEATLNEMFREVEELMEDTQHKLRSAVEEMEAEEAAAKTSSEVNLASLPPNYHNETSTETRVGNNTVHVHQEVHKITNNQSGQVVFSETVITSVGDEEGKRSHECIIDEDCGPTRYCQFSSFKYTCQPCRDQQMLCTRDSECCGDQLCAWGHCTQKATKGGNGTICDNQRDCQPGLCCAFQRGLLFPVCTPLPVEGELCHDPTSQLLDLITWELEPEGALDRCPCASGLLCQPHSHSLVYMCKPAFVGSHDHSEESQLPREAPDEYEDVGFIGEVRQELEDLERSLAQEMAFEGPAPVESLGGEEEI

Approximately 60-66 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.