DNMT2 Protein, Human (sf9, GST)

The DNMT2 protein exhibits remarkable specificity by selectively methylating cytosine 38 within the anticodon loop of tRNA (Asp). This site-specific methylation is critical for epigenetic modification of transfer RNA, affecting its structure, stability, and functional interactions. DNMT2 Protein, Human (sf9, GST) is the recombinant human-derived DNMT2 protein, expressed by Sf9 insect cells , with N-GST labeled tag. The total length of DNMT2 Protein, Human (sf9, GST) is 391 a.a., with molecular weight of ~60 kDa.

DNMT2 protein exhibits remarkable specificity in its enzymatic activity, as it selectively methylates cytosine 38 within the anticodon loop of tRNA(Asp). This site-specific methylation event plays a crucial role in the epigenetic modification of transfer RNA molecules, potentially influencing their structure, stability, and functional interactions. By targeting a specific cytosine residue in the anticodon loop of tRNA(Asp), DNMT2 contributes to the modulation of translational efficiency and fidelity. The enzyme's precision in methylating this particular site underscores its significance in the intricate regulatory mechanisms governing tRNA function, emphasizing the importance of DNMT2 in the epigenetic landscape of cellular processes involving tRNA(Asp).

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

Sf9 insect cells

N-GST

O14717 (M1-E391)

1787  [NCBI]

Approximately 60 kDa(Predicted Molecular Mass :70.88 kDa)

Greater than 90% as determined by reducing SDS-PAGE

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 0.5 mM GSH, 10% Glycerol, pH 8, 5% Trehalose, 5% Mannitol, 0.01% Tween-80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.