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DST Protein, Human (P.pastoris, His)

Cat. No.: HY-P71840
Handling Instructions

DST proteins integrate intermediate filaments, actin, and microtubule networks. DST Protein, Human (P.pastoris, His) is the recombinant human-derived DST protein, expressed by P. pastoris , with N-His labeled tag. The total length of DST Protein, Human (P.pastoris, His) is 195 a.a., with molecular weight of ~23.7 kDa.

For research use only. We do not sell to patients.

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Description

DST proteins integrate intermediate filaments, actin, and microtubule networks. DST Protein, Human (P.pastoris, His) is the recombinant human-derived DST protein, expressed by P. pastoris , with N-His labeled tag. The total length of DST Protein, Human (P.pastoris, His) is 195 a.a., with molecular weight of ~23.7 kDa.

Background

DST Protein serves as a crucial cytoskeletal linker, integrating intermediate filaments, actin, and microtubule cytoskeleton networks. Its role varies across cell types, anchoring intermediate filaments to the actin cytoskeleton in neural and muscle cells or connecting keratin-containing intermediate filaments to hemidesmosomes in epithelial cells. DST Protein may self-aggregate to form filaments or a two-dimensional mesh. In sensory neurons, it regulates the organization and stability of the microtubule network, facilitating axonal transport. Additionally, it mediates the docking of the dynein/dynactin motor complex to vesicle cargos for retrograde axonal transport through interactions with TMEM108 and DCTN1. In epithelial cells, DST Protein plays a structural role in assembling hemidesmosomes, anchoring keratin-containing intermediate filaments to the inner plaque and contributing to the regulation of keratinocyte polarity and motility, particularly in mediating integrin ITGB4's control of RAC1 activity.

Species

Human

Source

P. pastoris

Tag

N-His

Accession

Q03001 (1M-195G)

Gene ID

667  [NCBI]

Molecular Construction
N-term
His
DST (1M-195G)
Accession # Q03001
C-term
Synonyms
DST; BPAG1; DMH; DT; Dystonia musculorum protein; Hemidesmosomal plaque protein
AA Sequence

MHSSSYSYRSSDSVFSNTTSTRTSLDSNENLLLVHCGPTLINSCISFGSESFDGHRLEMLQQIANRVQRDSVICEDKLILAGNALQSDSKRLESGVQFQNEAEIAGYILECENLLRQHVIDVQILIDGKYYQADQLVQRVAKLRDEIMALRNECSSVYSKGRILTTEQTKLMISGITQSLNSGFAQTLHPSLTSG

Molecular Weight

Approximately 23.7 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

DST Protein, Human (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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DST Protein, Human (P.pastoris, His)
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HY-P71840
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