DST Protein, Human (P.pastoris, His)

DST proteins integrate intermediate filaments, actin, and microtubule networks. DST Protein, Human (P.pastoris, His) is the recombinant human-derived DST protein, expressed by P. pastoris , with N-His labeled tag. The total length of DST Protein, Human (P.pastoris, His) is 195 a.a., with molecular weight of ~23.7 kDa.

DST Protein serves as a crucial cytoskeletal linker, integrating intermediate filaments, actin, and microtubule cytoskeleton networks. Its role varies across cell types, anchoring intermediate filaments to the actin cytoskeleton in neural and muscle cells or connecting keratin-containing intermediate filaments to hemidesmosomes in epithelial cells. DST Protein may self-aggregate to form filaments or a two-dimensional mesh. In sensory neurons, it regulates the organization and stability of the microtubule network, facilitating axonal transport. Additionally, it mediates the docking of the dynein/dynactin motor complex to vesicle cargos for retrograde axonal transport through interactions with TMEM108 and DCTN1. In epithelial cells, DST Protein plays a structural role in assembling hemidesmosomes, anchoring keratin-containing intermediate filaments to the inner plaque and contributing to the regulation of keratinocyte polarity and motility, particularly in mediating integrin ITGB4's control of RAC1 activity.

Human

P. pastoris

N-His

Q03001 (1M-195G)

667  [NCBI]

MHSSSYSYRSSDSVFSNTTSTRTSLDSNENLLLVHCGPTLINSCISFGSESFDGHRLEMLQQIANRVQRDSVICEDKLILAGNALQSDSKRLESGVQFQNEAEIAGYILECENLLRQHVIDVQILIDGKYYQADQLVQRVAKLRDEIMALRNECSSVYSKGRILTTEQTKLMISGITQSLNSGFAQTLHPSLTSG

Approximately 23.7 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.