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EED Protein, Human (sf9, His-GST)

Cat. No.: HY-P74179
Handling Instructions

EED protein is a core member of the Polycomb group (PcG) and is critical in the PRC2/EED-EZH2 complex, driving "Lys-9" and "Lys-27" methylation on histone H3 to achieve transcriptional repression . It recognizes trimethylated "Lys-26" on histone H1 and inhibits the methyltransferase activity of the PRC2 complex on nucleosomal histone H3 "Lys-27". EED Protein, Human (sf9, His-GST) is the recombinant human-derived EED protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of EED Protein, Human (sf9, His-GST) is 441 a.a., with molecular weight of 75-85 kDa.

For research use only. We do not sell to patients.

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Description

EED protein is a core member of the Polycomb group (PcG) and is critical in the PRC2/EED-EZH2 complex, driving "Lys-9" and "Lys-27" methylation on histone H3 to achieve transcriptional repression . It recognizes trimethylated "Lys-26" on histone H1 and inhibits the methyltransferase activity of the PRC2 complex on nucleosomal histone H3 "Lys-27". EED Protein, Human (sf9, His-GST) is the recombinant human-derived EED protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of EED Protein, Human (sf9, His-GST) is 441 a.a., with molecular weight of 75-85 kDa.

Background

EED, a Polycomb group (PcG) protein, serves as a key component of the PRC2/EED-EZH2 complex, contributing to the methylation of 'Lys-9' and 'Lys-27' residues on histone H3, resulting in the transcriptional repression of target genes. It plays a crucial role in recognizing trimethylated 'Lys-26' of histone H1, inhibiting the methyltransferase activity of the PRC2 complex on nucleosomal histone H3 'Lys-27'. Conversely, recognition of 'Lys-27' trimethylation enables the propagation of this repressive mark. Beyond its role in histone modification, the PRC2/EED-EZH2 complex may act as a platform recruiting DNA methyltransferases, bridging two distinct epigenetic repression systems. Genes subject to repression by this complex include HOXC8, HOXA9, MYT1, and CDKN2A. Comprising EED, EZH2, SUZ12, RBBP4, and RBBP7, and possibly AEBP2, the PRC2/EED-EZH2 complex requires EED, EZH2, and SUZ12 as minimum components for its methyltransferase activity. Furthermore, EED participates in the PRC2/EED-EZH1 complex, which involves EED, EZH1, SUZ12, RBBP4, and AEBP2. The intricate network of interactions, including those with histones, DNMTs, and other proteins, highlights EED's pivotal role in epigenetic regulation.

Species

Human

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

O75530 (M1-R441)

Gene ID
Molecular Construction
N-term
His-GST
EED (M1-R441)
Accession # O75530
C-term
Synonyms
EED; HEED; Polycomb protein EED; WAIT1
Molecular Weight

75-85 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

EED Protein, Human (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
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The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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EED Protein, Human (sf9, His-GST)
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HY-P74179
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