Enolase 1/ENO1 Protein, Rat (His)

Enolase 1 (ENO1) plays a key role in carbohydrate degradation, particularly in the glycolytic pathway, where it catalyzes the conversion of 2-phospho-D-glycerate to phosphoenolpyruvate.This enzyme activity occurs during the fourth step of glycolysis and marks a critical stage in the conversion of glucose to pyruvate.Enolase 1/ENO1 Protein, Rat (His) is the recombinant rat-derived Enolase 1/ENO1 protein, expressed by E.coli , with N-His labeled tag.

Enolase 1 (ENO1) is a key enzyme involved in carbohydrate degradation, specifically within the glycolytic pathway, where it catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. This reaction occurs in the penultimate steps of glycolysis and is crucial for the generation of pyruvate from D-glyceraldehyde 3-phosphate. ENO1 plays a central role in cellular energy metabolism by participating in the glycolytic process, converting intermediates into pyruvate, which can further enter various metabolic pathways. The enzymatic activity of ENO1 contributes to the production of ATP, making it an essential component of energy production in cells. Serval researches indicateENO1's involvement in the specific biochemical transformation from D-glyceraldehyde 3-phosphate to pyruvate during glycolysis, emphasizing its significance in cellular energy metabolism.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Rat

E. coli

N-His

Q5BJ93 (M1-K434)

24333  [NCBI]

Approximately 49.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 10% Glycerol, pH 8.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.