Eotaxin/CCL11 Protein, Human

Eotaxin/CCL11 Protein, Human is a potent chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia.

CCL11 is a small cytokine belonging to the CC chemokine family. CCL11 selectively recruits eosinophils by inducing their chemotaxis, and therefore, is implicated in allergicresponses^[1]. It is produced by IFN-γ-stimulated endothelial cells and TNF-activated monocytes. CCL11 is a potent chemoattractant for eosinophils, basophils, and Th2 lymphocytes. CCL11 has a number of important biological functions in disease processes. It plays a critical role in allergic and nonallergic inflammatory reactions, such as mycobacterial and schistosomal induced granulomatosis^[2].

CCL11 (0.1-100 nM, 48 h) can induce HMEC chemotaxis in a dose-dependent manner and can be blocked by 20 μg/mL of CCL11 mAb. However, CCL11 does not induce HMEC proliferation in vitro^[3].
CCL11 (1-100 ng) can induce angiogenesis, with 78% at 100 ng and 63% at 10 ng but no significant angiogenic response was obtained at a dose of 1 ng in the chick CAM assay^[3].

Full biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration range of 0.1-10.0 ng/ml.

Human

E. coli

Tag Free

P51671 (G24-P97)

6356  [NCBI]

GPASVPTTCCFNLANRKIPLQRLESYRRITSGKCPQKAVIFKTKLAKDICADPKKKWVQDSMKYLDQKSPTPKP

Approximately 8.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against 20 mM PB, pH 7.4, 150 mM NaCl.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Antonio L. Teixeira, et al. Revisiting the Role of Eotaxin-1/CCL11 in Psychiatric Disorders. Front Psychiatry. 2018 Jun 14;9:241.  [Content Brief]

  [2]. Garcia-Zepeda EA, et al. Human eotaxin is a specific chemoattractant for eosinophil cells and provides a new mechanism to explain tissue eosinophilia. Nat Med. 1996 Apr;2(4):449-56.  [Content Brief]

  [3]. R Salcedo, et al. Eotaxin (CCL11) induces in vivo angiogenic responses by human CCR3+ endothelial cells. J Immunol. 2001 Jun 15;166(12):7571-8.  [Content Brief]

  [4]. Salcedo R, et al. Eotaxin (CCL11) induces in vivo angiogenic responses by human CCR3+ endothelial cells. J Immunol. 2001 Jun 15;166(12):7571-8.  [Content Brief]

  [5]. Sara S Cheng, et al. Eotaxin/CCL11 is a negative regulator of neutrophil recruitment in a murine model of endotoxemia.Exp Mol Pathol. 2002 Aug;73(1):1-8.  [Content Brief]