ERO1L Protein, Human (HEK293, His)

ERO1L protein is an oxidoreductase that can effectively reoxidize P4HB/PDI in the endoplasmic reticulum and promote the formation of disulfide bonds. It transfers electrons to molecular oxygen, producing reactive oxygen species (ROS). ERO1L Protein, Human (HEK293, His) is the recombinant human-derived ERO1L protein, expressed by HEK293 , with C-6*His labeled tag. The total length of ERO1L Protein, Human (HEK293, His) is 445 a.a., with molecular weight of ~71.0 kDa.

ERO1L protein functions as an oxidoreductase, facilitating disulfide bond formation within the endoplasmic reticulum (ER). Its primary role involves efficiently reoxidizing P4HB/PDI, an enzyme crucial for catalyzing protein disulfide formation, enabling P4HB to engage in successive rounds of disulfide bond formation. Following the reoxidation of P4HB, ERO1L transfers electrons to molecular oxygen through its flavin adenine dinucleotide (FAD) cofactor, leading to the generation of reactive oxygen species (ROS) within the cell. This protein is essential for the proper folding of immunoglobulins and plays a significant role in ER stress-induced apoptosis by activating the inositol 1,4,5-trisphosphate receptor IP3R1, particularly in a CHOP-dependent manner. Additionally, ERO1L participates in the release of unfolded cholera toxin during V. cholerae infection, contributing to the retrotranslocation of the toxin.

Human

HEK293

C-6*His

Q96HE7 (E24-H468)

30001  [NCBI]

EEQPPETAAQRCFCQVSGYLDDCTCDVETIDRFNNYRLFPRLQKLLESDYFRYYKVNLKRPCPFWNDISQCGRRDCAVKPCQSDEVPDGIKSASYKYSEEANNLIEECEQAERLGAVDESLSEETQKAVLQWTKHDDSSDNFCEADDIQSPEAEYVDLLLNPERYTGYKGPDAWKIWNVIYEENCFKPQTIKRPLNPLASGQGTSEENTFYSWLEGLCVEKRAFYRLISGLHASINVHLSARYLLQETWLEKKWGHNITEFQQRFDGILTEGEGPRRLKNLYFLYLIELRALSKVLPFFERPDFQLFTGNKIQDEENKMLLLEILHEIKSFPLHFDENSFFAGDKKEAHKLKEDFRLHFRNISRIMDCVGCFKCRLWGKLQTQGLGTALKILFSEKLIANMPESGPSYEFHLTRQEIVSLFNAFGRISTSVKELENFRNLLQNIH

Approximately 71.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.