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  2. Immune Checkpoint Proteins
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  4. FGL-1
  5. FGL1 Protein, Mouse (HEK293, hFc)

FGL1 Protein, Mouse (HEK293, hFc)

Cat. No.: HY-P75189
COA Handling Instructions

The FGL1 protein has signaling receptor binding activity and negatively regulates T cell activation and immune response. It is involved in adipose tissue development, cholesterol metabolism, and response to stilbenoid. FGL1 is located in the extracellular region and is orthologous to the human FGL1 gene. It is primarily expressed in the liver at the E18 stage. FGL1 Protein, Mouse (HEK293, hFc) is the recombinant mouse-derived FGL1 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
10 μg $72 In-stock
20 μg $115 In-stock
50 μg $220 In-stock
100 μg $350 In-stock
500 μg $980 In-stock
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  • Biological Activity

  • Technical Parameters

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Description

The FGL1 protein has signaling receptor binding activity and negatively regulates T cell activation and immune response. It is involved in adipose tissue development, cholesterol metabolism, and response to stilbenoid. FGL1 is located in the extracellular region and is orthologous to the human FGL1 gene. It is primarily expressed in the liver at the E18 stage. FGL1 Protein, Mouse (HEK293, hFc) is the recombinant mouse-derived FGL1 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

The FGL1 protein is predicted to have signaling receptor binding activity and is involved in the negative regulation of T cell activation and immune response. It plays a role upstream or within various processes, including adipose tissue development, cholesterol metabolic process, and response to stilbenoid. The protein is located in the extracellular region and is orthologous to the human FGL1 (fibrinogen like 1) gene. Additionally, it exhibits restricted expression primarily in the liver at E18 stage.

Biological Activity

Immobilized Mouse LAG-3, hFc Tag (HY-P73843) at 5 μg/mL (100 μL/well) can bind Mouse FGL1. The ED50 for this effect is 0.5343 μg/mL, corresponding to a specific activity is 1.87×103 Unit/mg.

  • Immobilized Mouse LAG-3, hFc Tag (HY-P73843) at 5 μg/mL (100 μL/well) can bind Mouse FGL1. The ED50 for this effect is 0.5343 μg/mL, corresponding to a specific activity is 1.87×103 Unit/mg.
Species

Mouse

Source

HEK293

Tag

C-hFc

Accession

NP_663569.2 (L23-I314)

Gene ID
Molecular Construction
N-term
FGL1 (L23-I314)
Accession # NP_663569.2
hFc
C-term
Synonyms
Fibrinogen-like protein 1; FGL1; HP-041; Hepassocin; HFREP-1; LFIRE-1
AA Sequence

LESENCLREQVRLRAQVHQLETRVKQQQTMIAQLLHEKEVQFLDKGSENSFIDLGGKKQYADCSEIYNDGFKQSGFYKIKPLQSLAEFSVYCDMSDGGGWTVIQRRSDGSENFNRGWNDYENGFGNFVQNNGEYWLGNKNINLLTIQGDYTLKIDLTDFEKNSSFAQYQSFKVGDKKSFYELNIGEYSGTAGDSLSGTFHPEVQWWASHQRMKFSTWDRDNDNYQGNCAEEEQSGWWFNRCHSANLNGVYYRGSYRAETDNGVVWYTWHGWWYSLKSVVMKIRPSDFIPNII

Molecular Weight

Approximately 60.08 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4 (Normally trehalose is added as protectant before lyophilization. ) or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

FGL1 Protein, Mouse (HEK293, hFc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FGL1 Protein, Mouse (HEK293, hFc)
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HY-P75189
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