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  4. FNDC4 Protein, Human (HEK293, Fc)

FNDC4 Protein, Human (HEK293, Fc)

Cat. No.: HY-P76935
COA Handling Instructions

The FNDC4 protein is an anti-inflammatory factor in the intestine and colon that regulates macrophages by downregulating pro-inflammatory gene expression and affecting phagocytosis. It does this by modulating key pathways associated with macrophage activation, in part through STAT3 activation and signaling. FNDC4 Protein, Human (HEK293, Fc) is the recombinant human-derived FNDC4 protein, expressed by HEK293 , with C-mFc labeled tag. The total length of FNDC4 Protein, Human (HEK293, Fc) is 123 a.a., with molecular weight of ~50-60 KDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

The FNDC4 protein is an anti-inflammatory factor in the intestine and colon that regulates macrophages by downregulating pro-inflammatory gene expression and affecting phagocytosis. It does this by modulating key pathways associated with macrophage activation, in part through STAT3 activation and signaling. FNDC4 Protein, Human (HEK293, Fc) is the recombinant human-derived FNDC4 protein, expressed by HEK293 , with C-mFc labeled tag. The total length of FNDC4 Protein, Human (HEK293, Fc) is 123 a.a., with molecular weight of ~50-60 KDa.

Background

FNDC4 protein functions as an anti-inflammatory factor in the intestine and colon, exerting its regulatory influence on macrophages. Through binding and interaction with macrophages, FNDC4 down-regulates the expression of pro-inflammatory genes, influencing essential macrophage functions such as phagocytosis. This anti-inflammatory effect is achieved by modulating key pathways associated with macrophage activation, in part through the activation and signaling of STAT3. The role of FNDC4 in dampening the immunological response, particularly in the context of colitis, underscores its potential significance as a molecular mediator in maintaining intestinal immune homeostasis.

Biological Activity

Measured in a cell proliferation assay using U87 cells. The ED50 for this effect is 63 ng/mL. Corresponding to a specific activity is 1.587×104 unit/mg.

  • Measured in a cell proliferation assay using U87 cells. The ED50 for this effect is 63 ng/mL. Corresponding to a specific activity is 1.587×104 Unit/mg.
Species

Human

Source

HEK293

Tag

C-mFc

Accession

Q9H6D8/NP_073734.1 (D45-T167)

Gene ID
Molecular Construction
N-term
FNDC4 (D45-T167)
Accession # Q9H6D8/NP_073734.1
mFc
C-term
Synonyms
Fibronectin type III domain-containing protein 4; Fibronectin type III repeat-containing protein 1; FRCP1
AA Sequence

DRPPSPVNVTVTHLRANSATVSWDVPEGNIVIGYSISQQRQNGPGQRVIREVNTTTRACALWGLAEDSDYTVQVRSIGLRGESPPGPRVHFRTLKGSDRLPSNSSSPGDITVEGLDGERPLQT

Molecular Weight

Approximately 50-60 kDa due to the glycosylation

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

FNDC4 Protein, Human (HEK293, Fc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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FNDC4 Protein, Human (HEK293, Fc)
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HY-P76935
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