Frizzled-5 Protein, Mouse (CHO, Fc)

Frizzled-5 is a Wnt receptor that activates canonical Wnt/β-catenin signaling through WNT2, WNT10B, and WNT5A. It participates in a potential second pathway involving PKC and calcium flux, affecting Wnt-mediated inactivation of GSK-3 kinase. Frizzled-5 Protein, Mouse (CHO, Fc) is the recombinant mouse-derived Frizzled-5 protein, expressed by CHO , with C-hFc labeled tag. The total length of Frizzled-5 Protein, Mouse (CHO, Fc) is 167 a.a., with molecular weight of 52-56 kDa.

Frizzled-5, a receptor for Wnt proteins, demonstrates a nuanced activation profile, being responsive to WNT2, WNT10B, and WNT5A, while showing no affinity for WNT2B or WNT4 in vitro. Its involvement in canonical Wnt/beta-catenin signaling is pivotal, leading to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin, and subsequent activation of Wnt target genes. Additionally, a second signaling pathway, potentially involving PKC and calcium fluxes, adds complexity, with implications for Wnt-mediated inactivation of GSK-3 kinase. Frizzled-5's engagement in tissue morphogenesis and potential participation in transducing polarity information underscores its versatile role. Notably, in neurons, WNT7A activation facilitates synapse formation. Its contributions extend to yolk sac angiogenesis and placental vascularization. Homodimerization, facilitated by binding of unsaturated fatty acid molecules, and interactions with WNT2B, WNT7A, and GOPC further underscore the multifaceted regulatory functions of Frizzled-5 within intricate signaling networks.

Mouse

CHO

C-hFc

Q9EQD0 (M1-P167)

14367  [NCBI]

52-56 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.