Fumarate Hydratase/FH Protein, Human (His)

The fumarate hydratase/FH protein plays a key role in cellular processes as it catalyzes the hydration of fumarate to L-malate in the tricarboxylic acid (TCA) cycle, which is essential for energy production through the production of NADH . Fumarate Hydratase/FH Protein, Human (His) is the recombinant human-derived Fumarate Hydratase/FH protein, expressed by E. coli , with C-His labeled tag. The total length of Fumarate Hydratase/FH Protein, Human (His) is 466 a.a., with molecular weight of ~50 kDa.

Fumarate Hydratase (FH) is an enzyme that catalyzes the reversible conversion of fumarate to L-malate. This stereospecific interconversion plays a pivotal role in the tricarboxylic acid (TCA) cycle, a central metabolic pathway involved in energy production. Specifically, FH facilitates the hydration of fumarate to L-malate, contributing to a key transition step in the TCA cycle. Experiments in various species suggest that specific isoforms of FH may act in defined pathways and exhibit preferences for one direction of the reaction over the other. By participating in the TCA cycle, FH ensures the efficient generation of energy in the form of NADH, highlighting its essential role in cellular metabolism.

Measured by its ability to transform 1 umole of Fumarate to L-malate per minute at pH 7.5 at 37°C. Specific activity is 22.89 U/mg.

Human

E. coli

C-His

P07954-1 (A45-K510)

2271  [NCBI]

ASQNSFRIEYDTFGELKVPNDKYYGAQTVRSTMNFKIGGVTERMPTPVIKAFGILKRAAAEVNQDYGLDPKIANAIMKAADEVAEGKLNDHFPLVVWQTGSGTQTNMNVNEVISNRAIEMLGGELGSKIPVHPNDHVNKSQSSNDTFPTAMHIAAAIEVHEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK

Approximately 50 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris, 5 mM EDTA, 1 mM DTT, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.