GBA/Glucosylceramidase Protein, Human (HEK293, His)

GBA (glucosylceramidase) is a lysosomal enzyme that degrades lipids and aids in cell membrane turnover. It hydrolyzes glucosylceramide to form ceramide and glucose. GBA/Glucosylceramidase Protein, Human (HEK293, His) is the recombinant human-derived GBA/Glucosylceramidase protein, expressed by HEK293 , with C-His labeled tag.

GBA (Glucosylceramidase) is a crucial enzyme located within the lysosomal compartment, playing a central role in the degradation of complex lipids and cellular membrane turnover. It catalyzes the hydrolysis of glucosylceramides (GlcCers), such as beta-D-glucosyl-(1<->1')-N-acylsphing-4-enine, into free ceramides and glucose. This enzymatic activity is essential for the PKC-activated salvage pathway of ceramide formation. Additionally, GBA participates in a transglucosylation reaction, transferring glucose from GlcCer to cholesterol, a process preferred with GlcCers containing mono-unsaturated fatty acids. Under specific conditions, GBA may catalyze the reverse reaction, transferring glucose from cholesteryl 3-beta-D-glucoside to ceramide. Furthermore, GBA can hydrolyze cholesteryl 3-beta-D-glucoside, yielding glucose and cholesterol. While GBA exhibits lower activity with galactosylceramides (GalCers), it can also transfer galactose between GalCers and cholesterol in vitro. In contrast, xylosylceramide (XylCer) is not a significant substrate for hydrolysis, but it serves as a good xylose donor for transxylosylation activity, forming cholesteryl 3-beta-D-xyloside.

Measured by its ability to hydrolyze 4-methylumbelliferyl-beta-D-glucopyranoside(HY-123633). The specific activity is 286- 542 pmol/min/μg that incubate at 37°C for 20 minutes.

Human

HEK293

C-His

P04062-1/NP_000148.2 (A40-Q536)

2629  [NCBI]

ARPCIPKSFGYSSVVCVCNATYCDSFDPPTFPALGTFSRYESTRSGRRMELSMGPIQANHTGTGLLLTLQPEQKFQKVKGFGGAMTDAAALNILALSPPAQNLLLKSYFSEEGIGYNIIRVPMASCDFSIRTYTYADTPDDFQLHNFSLPEEDTKLKIPLIHRALQLAQRPVSLLASPWTSPTWLKTNGAVNGKGSLKGQPGDIYHQTWARYFVKFLDAYAEHKLQFWAVTAENEPSAGLLSGYPFQCLGFTPEHQRDFIARDLGPTLANSTHHNVRLLMLDDQRLLLPHWAKVVLTDPEAAKYVHGIAVHWYLDFLAPAKATLGETHRLFPNTMLFASEACVGSKFWEQSVRLGSWDRGMQYSHSIITNLLYHVVGWTDWNLALNPEGGPNWVRNFVDSPIIVDITKDTFYKQPMFYHLGHFSKFIPEGSQRVGLVASQKNDLDAVALMHPDGSAVVVVLNRSSKDVPLTIKDPAVGFLETISPGYSIHTYLWRRQ

Approximately 60-80 kDa due to the glycosylation.

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or PBS, pH 8.0, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.