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GBP1 Protein, Human (HEK293, His)

Cat. No.: HY-P75172
COA Handling Instructions

GBP1 Protein belongs to the dynamin superfamily of large GTPases. GBP1 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. It hydrolyzes GTP to GMP in two consecutive cleavage reactions. GBP1 also acts as a positive regulator of inflammasome assembly by promoting the release of inflammasome ligands from bacteria. In addition, it exhibits antiviral activity against influenza virus. GBP1 Protein, Human (HEK293, His) is the recombinant human-derived GBP1 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of GBP1 Protein, Human (HEK293, His) is 589 a.a., with molecular weight of ~65 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
2 μg $42 In-stock
10 μg $115 In-stock
20 μg $185 In-stock
50 μg $350 In-stock
100 μg   Get quote  

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  • Biological Activity

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Description

GBP1 Protein belongs to the dynamin superfamily of large GTPases. GBP1 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. It hydrolyzes GTP to GMP in two consecutive cleavage reactions. GBP1 also acts as a positive regulator of inflammasome assembly by promoting the release of inflammasome ligands from bacteria. In addition, it exhibits antiviral activity against influenza virus. GBP1 Protein, Human (HEK293, His) is the recombinant human-derived GBP1 protein, expressed by HEK293 , with C-10*His labeled tag. The total length of GBP1 Protein, Human (HEK293, His) is 589 a.a., with molecular weight of ~65 kDa.

Background

Guanylate-binding protein 1 (GBP1) belongs to the dynamin superfamily of large GTPases. GBP1 plays important roles in innate immunity against a diverse range of bacterial, viral and protozoan pathogens. It hydrolyzes GTP to GMP in two consecutive cleavage reactions: GTP is first hydrolyzed to GDP and then to GMP in a processive manner. Following infection, GBP1 is recruited to the pathogen-containing vacuoles or vacuole-escaped bacteria and promotes both autophagy and inflammasome assembly. GBP1 also acts as a positive regulator of inflammasome assembly by promoting the release of inflammasome ligands from bacteria. GBP1 confers protection to several pathogens, including the bacterial pathogens L.monocytogenes and M.bovis BCG as well as the protozoan pathogen T.gondii. In addition, it exhibits antiviral activity against influenza virus[1][2].

Species

Human

Source

HEK293

Tag

C-10*His

Accession

AAA35871.1 (M1-C589)

Gene ID
Molecular Construction
N-term
GBP1 (M1-C589)
Accession # AAA35871.1
10*His
C-term
Synonyms
Guanylate-binding protein 1; GBP-1; HuGBP-1
Molecular Weight

Approximately 65 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

GBP1 Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
GBP1 Protein, Human (HEK293, His)
Cat. No.:
HY-P75172
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