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  6. GFRAL Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)

GFRAL Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P700997
Handling Instructions

The GFRAL protein is a brainstem-restricted receptor for GDF15 that regulates food intake, energy expenditure, and body weight in response to metabolic and toxin-induced stress. GFRAL binds to its ligand GDF15, interacts with RET, and activates the MAPK and AKT signaling pathways. GFRAL Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) is the recombinant cynomolgus-derived GFRAL protein, expressed by HEK293 , with C-Avi, N-His labeled tag. The total length of GFRAL Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) is 332 a.a., with molecular weight of 50-70 kDa.

For research use only. We do not sell to patients.

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Description

The GFRAL protein is a brainstem-restricted receptor for GDF15 that regulates food intake, energy expenditure, and body weight in response to metabolic and toxin-induced stress. GFRAL binds to its ligand GDF15, interacts with RET, and activates the MAPK and AKT signaling pathways. GFRAL Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) is the recombinant cynomolgus-derived GFRAL protein, expressed by HEK293 , with C-Avi, N-His labeled tag. The total length of GFRAL Protein, Cynomolgus (Biotinylated, HEK293, His-Avi) is 332 a.a., with molecular weight of 50-70 kDa.

Background

GFRAL Protein, a brainstem-restricted receptor for GDF15, plays a crucial role in regulating food intake, energy expenditure, and body weight in response to metabolic and toxin-induced stresses. Upon binding to its ligand, GDF15, GFRAL interacts with RET and activates cellular signaling through the MAPK- and AKT-signaling pathways. The receptor, through its extracellular domain, forms complexes with both GDF15 and RET, mediating cellular signaling specifically when RET is engaged after GDF15 binding. This intricate interaction highlights the sequential steps involving GFRAL, GDF15, and RET in the modulation of physiological responses to metabolic challenges.

Species

Cynomolgus

Source

HEK293

Tag

N-Avi;N-His

Accession

XP_015304775.2 (Q20-E351)

Gene ID
Molecular Construction
N-term
His-Avi
GFRAL (Q20-E351)
Accession # XP_015304775.2
C-term
Synonyms
GFR alpha-like; GFRAL; GRAL; C6orf144
Molecular Weight

50-70 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
GFRAL Protein, Cynomolgus (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P700997
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