GMP IL-18 Protein, Human

GMP IL-18 protein is a pro-inflammatory cytokine that plays a critical role in epithelial barrier repair and polarized immune responses involving Th1 cells and NK cells. After binding to IL18R1 and IL18RAP, it forms a signaling ternary complex that activates NF-kappa-B, leading to the synthesis of inflammatory mediators. GMP IL-18 Protein, Human is the recombinant human-derived GMP IL-18 protein, expressed by E. coli , with tag free.

GMP IL-18 Protein is a pro-inflammatory cytokine with pivotal roles in epithelial barrier repair and orchestrating polarized immune responses involving T-helper 1 (Th1) cells and natural killer (NK) cells. Upon binding to IL18R1 and IL18RAP, IL-18 forms a signaling ternary complex that activates NF-kappa-B, leading to the synthesis of inflammatory mediators. It synergizes with IL-12 to induce IFNG synthesis in Th1 cells and NK cells. Notably, GMP IL-18 is involved in transduction of inflammation downstream of pyroptosis, where its mature form is released into the extracellular milieu by passing through the gasdermin-D (GSDMD) pore. At the plasma membrane, IL-18 forms a ternary complex with the ligand-binding receptor subunit IL18R1 and signaling receptor subunit IL18RAP, initiating intracellular signaling. Additionally, IL-18 interacts with the cargo receptor TMED10, facilitating its translocation from the cytoplasm into the endoplasmic reticulum-Golgi intermediate compartment (ERGIC) and subsequent secretion.

Human

E. coli

Tag Free

Q14116-1 (Y37-D193)

3606

YFGKLESKLSVIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIISMYKDSQPRGMAVTISVKCEKISTLSCENKIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLFKLILKKEDELGDRSIMFTVQNED

Approximately 18.2 kDa

Greater than 95% as determined by reducing SDS-PAGE.

<0.01 EU/μg, determined by LAL method.