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Grancalcin/GCA Protein, Human (His)

Cat. No.: HY-P70334
COA Handling Instructions

Grancalcin/GCA protein is a calcium-binding protein that plays a potential role in neutrophil adhesion to fibronectin and focal adhesion formation.As a homodimer, this protein exhibits interactions with SRI and LCP1, suggesting its involvement in molecular pathways related to cell adhesion and cytoskeletal dynamics.Grancalcin/GCA Protein, Human (His) is the recombinant human-derived Grancalcin/GCA protein, expressed by E.coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.

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Description

Grancalcin/GCA protein is a calcium-binding protein that plays a potential role in neutrophil adhesion to fibronectin and focal adhesion formation.As a homodimer, this protein exhibits interactions with SRI and LCP1, suggesting its involvement in molecular pathways related to cell adhesion and cytoskeletal dynamics.Grancalcin/GCA Protein, Human (His) is the recombinant human-derived Grancalcin/GCA protein, expressed by E.coli , with N-6*His labeled tag.

Background

Grancalcin (GCA) is a calcium-binding protein with putative functions in neutrophil adhesion to fibronectin and the formation of focal adhesions. Its role in calcium binding suggests involvement in signaling pathways that regulate cellular adhesion processes. GCA is known to form homodimers and has interactions with SRI and LCP1, further implicating its participation in protein-protein interactions that could modulate cellular functions. It has to emphasize GCA's potential involvement in mediating neutrophil adhesion to fibronectin and suggests a role in the establishment of focal adhesions, shedding light on its significance in cellular adhesion and signaling pathways.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

P28676 (M1-I217)

Gene ID
Molecular Construction
N-term
6*His
GCA (M1-I217)
Accession # P28676
C-term
Synonyms
rHuGrancalcin/GCA; Grancalcin; GCA; GCL
AA Sequence

MAYPGYGGGFGNFSIQVPGMQMGQPVPETGPAILLDGYSGPAYSDTYSSAGDSVYTYFSAVAGQDGEVDAEELQRCLTQSGINGTYSPFSLETCRIMIAMLDRDHTGKMGFNAFKELWAALNAWKENFMTVDQDGSGTVEHHELRQAIGLMGYRLSPQTLTTIVKRYSKNGRIFFDDYVACCVKLRALTDFFRKRDHLQQGSANFIYDDFLQGTMAI

Molecular Weight

Approximately 25 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 200 mM NaCl, 100 mM arginine, pH 8.0.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US;may vary elsewhere.

Documentation

Grancalcin/GCA Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Grancalcin/GCA Protein, Human (His)
Cat. No.:
HY-P70334
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