HARS/HisRS Protein, Human (sf9, His)

HARS is a key enzyme that coordinates the ATP-dependent attachment of histidine to the 3' end of its corresponding tRNA through the formation of an aminoacyl adenylate intermediate (His-AMP). This critical step in protein synthesis ensures the accurate incorporation of histidine into the nascent polypeptide chain. HARS/HisRS Protein, Human (sf9, His) is the recombinant human-derived HARS/HisRS protein, expressed by Sf9 insect cells , with N-His labeled tag.

HARS (Histidyl-tRNA synthetase), also known as HisRS, is a crucial enzyme that catalyzes the ATP-dependent ligation of histidine to the 3'-end of its corresponding tRNA, forming an aminoacyl-adenylate intermediate known as His-AMP. This process is essential for the accurate incorporation of histidine into growing polypeptide chains during protein synthesis. Beyond its canonical role in translation, HARS has been implicated in axon guidance, suggesting an additional function in cellular processes beyond protein biosynthesis. It has to highlight the enzyme's pivotal role in histidine activation and its intriguing involvement in axon guidance, broadening our understanding of its potential contributions to both translation and neuronal development.

Human

Sf9 insect cells

N-His

P12081-1/NP_002100.2 (A2-C509)

3035  [NCBI]

Approximately 59.6 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.