HDAC6 Protein, Human (His)

The HDAC6 protein coordinates multiple functions, deacetylating histones and proteins such as tubulin and CTTN. Its role in epigenetic repression affects transcription, cell cycle, and development. HDAC6 Protein, Human (His) is the recombinant human-derived HDAC6 protein, expressed by E. coli , with N-6*His labeled tag. The total length of HDAC6 Protein, Human (His) is 352 a.a., with molecular weight of ~42.4 kDa.

The HDAC6 protein assumes a multifaceted role, primarily responsible for the deacetylation of lysine residues on the N-terminal portion of core histones (H2A, H2B, H3, and H4), contributing to the epigenetic repression that plays crucial roles in transcriptional regulation, cell cycle progression, and developmental events. Operating within large multiprotein complexes, histone deacetylases, including HDAC6, orchestrate these processes. Beyond histones, HDAC6 extends its deacetylation activity to diverse proteins, such as tubulin, essential for microtubule-dependent cell motility, and alpha-tubulin, pivotal for cilia disassembly. Additionally, HDAC6 facilitates actin polymerization and autophagosome-lysosome fusion by deacetylating CTTN, promoting autophagy completion. Its involvement in the MTA1-mediated epigenetic regulation of ESR1 expression in breast cancer and promotion of odontoblast differentiation showcase its diverse functions. Furthermore, HDAC6 plays a key role in the degradation of misfolded proteins, acting as an adapter to recognize polyubiquitinated misfolded proteins and targeting them to the aggresome for clearance via autophagy when the chaperone refolding system and the ubiquitin-proteasome are insufficient.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-6*His

Q9UBN7-1 (M489-E840)

10013  [NCBI]

MMNHCNLWDSHHPEVPQRILRIMCRLEELGLAGRCLTLTPRPATEAELLTCHSAEYVGHLRATEKMKTRELHRESSNFDSIYICPSTFACAQLATGAACRLVEAVLSGEVLNGAAVVRPPGHHAEQDAACGFCFFNSVAVAARHAQTISGHALRILIVDWDVHHGNGTQHMFEDDPSVLYVSLHRYDHGTFFPMGDEGASSQIGRAAGTGFTVNVAWNGPRMGDADYLAAWHRLVLPIAYEFNPELVLVSAGFDAARGDPLGGCQVSPEGYAHLTHLLMGLASGRIILILEGGYNLTSISESMAACTRSLLGDPPPLLTLPRPPLSGALASITETIQVHRRYWRSLRVMKVE

Approximately 42.4 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.