Hepcidin/HAMP Protein, Mouse (GST)

The hepcidin/HAMP protein is a liver-produced hormone that is a major regulator of iron absorption and distribution. It promotes endocytosis and degradation of SLC40A1, retaining iron within cells and reducing plasma iron flux. Hepcidin/HAMP Protein, Mouse (GST) is the recombinant mouse-derived Hepcidin/HAMP protein, expressed by E. coli , with N-GST labeled tag.

Hepcidin, a liver-produced hormone, stands as the principal circulating regulator governing the absorption and distribution of iron throughout the body. Its regulatory mechanism involves the promotion of endocytosis and degradation of SLC40A1, resulting in the retention of iron within iron-exporting cells and a consequential reduction in iron flow into the plasma. Hepcidin orchestrates vital iron fluxes, including the absorption of dietary iron in the intestine, the recycling of iron by macrophages through phagocytosis of aging erythrocytes and other cells, and the mobilization of stored iron from hepatocytes. Its interaction with SLC40A1 triggers rapid ubiquitination of the latter, underscoring hepcidin's pivotal role in maintaining iron homeostasis.

Measured by its binding ability in a functional ELISA. Immobilized HAMP at 10μg/mL (100μL/well) can bind Anti-HAMP antibody. The ED₅₀ for this effect is 1.303 μg/mL.

Mouse

E. coli

N-GST

Q9EQ21 (D59-T83)

84506  [NCBI]

DTNFPICIFCCKCCNNSQCGICCKT

Predict MW: 28.24 kDa; Approximately 27 kDa band in SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.