HK1/Hexokinase-1 Protein, Human (P. pastoris, His)

The HK1/hexokinase-1 protein phosphorylates hexose sugars, including D-glucose, D-glucosamine, D-fructose, D-mannose, and 2-deoxy-D-glucose, to form the corresponding hexose 6-phosphate. HK1/Hexokinase-1 Protein, Human (P. pastoris, His) is the recombinant human-derived HK1/Hexokinase-1 protein, expressed by P. pastoris , with N-6*His labeled tag.

The Hexokinase-1 (HK1) protein plays a pivotal role in cellular metabolism by catalyzing the phosphorylation of various hexoses, including D-glucose, D-glucosamine, D-fructose, D-mannose, and 2-deoxy-D-glucose, to generate hexose 6-phosphate (D-glucose 6-phosphate, D-glucosamine 6-phosphate, D-fructose 6-phosphate, D-mannose 6-phosphate, and 2-deoxy-D-glucose 6-phosphate, respectively). While HK1 does not phosphorylate N-acetyl-D-glucosamine, it mediates the critical initial step of glycolysis by catalyzing the phosphorylation of D-glucose to D-glucose 6-phosphate. Beyond its metabolic functions, HK1 is involved in innate immunity and inflammation, acting as a pattern recognition receptor for bacterial peptidoglycan. In the cytosol, the N-acetyl-D-glucosamine component of bacterial peptidoglycan inhibits HK1's activity, leading to its dissociation from the mitochondrial outer membrane and subsequently activating the NLRP3 inflammasome. This multifaceted role underscores HK1's significance in cellular processes beyond glycolysis, highlighting its involvement in immune response and inflammation.

Human

P. pastoris

N-6*His

P19367-1 (E13-A475)

3098  [NCBI]

ELKDDQVKKIDKYLYAMRLSDETLIDIMTRFRKEMKNGLSRDFNPTATVKMLPTFVRSIPDGSEKGDFIALDLGGSSFRILRVQVNHEKNQNVHMESEVYDTPENIVHGSGSQLFDHVAECLGDFMEKRKIKDKKLPVGFTFSFPCQQSKIDEAILITWTKRFKASGVEGADVVKLLNKAIKKRGDYDANIVAVVNDTVGTMMTCGYDDQHCEVGLIIGTGTNACYMEELRHIDLVEGDEGRMCINTEWGAFGDDGSLEDIRTEFDREIDRGSLNPGKQLFEKMVSGMYLGELVRLILVKMAKEGLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILTRLGVEPSDDDCVSVQHVCTIVSFRSANLVAATLGAILNRLRDNKGTPRLRTTVGVDGSLYKTHPQYSRRFHKTLRRLVPDSDVRFLLSESGSGKGAAMVTAVAYRLAEQHRQIEETLA

53.9 kDa

Greater than 90% as determined by reducing SDS-PAGE.

<1 EU/μg, determined by LAL method.