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  4. HK3/Hexokinase-3 Protein, Human (sf9, His-GST)

HK3/Hexokinase-3 Protein, Human (sf9, His-GST)

Cat. No.: HY-P74881
Handling Instructions

The HK3 (hexokinase-3) protein promotes the phosphorylation of hexoses (i.e., D-glucose and D-fructose) to form hexose-6-phosphate (specifically, D-glucose 6-phosphate and 6-phosphate), Plays a vital role in cell metabolism. D-fructose 6-phosphate. HK3/Hexokinase-3 Protein, Human (sf9, His-GST) is the recombinant human-derived HK3/Hexokinase-3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of HK3/Hexokinase-3 Protein, Human (sf9, His-GST) is 923 a.a., with molecular weight of ~115 kDa.

For research use only. We do not sell to patients.

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Description

The HK3 (hexokinase-3) protein promotes the phosphorylation of hexoses (i.e., D-glucose and D-fructose) to form hexose-6-phosphate (specifically, D-glucose 6-phosphate and 6-phosphate), Plays a vital role in cell metabolism. D-fructose 6-phosphate. HK3/Hexokinase-3 Protein, Human (sf9, His-GST) is the recombinant human-derived HK3/Hexokinase-3 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag. The total length of HK3/Hexokinase-3 Protein, Human (sf9, His-GST) is 923 a.a., with molecular weight of ~115 kDa.

Background

The HK3 protein, also known as Hexokinase-3, plays a crucial role in glycolysis by catalyzing the phosphorylation of hexoses, including D-glucose and D-fructose, to form hexose 6-phosphates such as D-glucose 6-phosphate and D-fructose 6-phosphate, respectively. This enzyme mediates the initial and rate-limiting step of glycolysis, facilitating the conversion of D-glucose to D-glucose 6-phosphate. Through this enzymatic activity, HK3 contributes to the entry of glucose into the glycolytic pathway, a fundamental process in cellular energy metabolism. The phosphorylation of hexoses by HK3 is essential for subsequent metabolic pathways and energy production within the cell.

Species

Human

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

P52790 (M1-V923)

Gene ID
Molecular Construction
N-term
His-GST
HK3 (M1-V923)
Accession # P52790
C-term
Synonyms
Hexokinase-3; HK III; Hexokinase-C; HK3
Molecular Weight

Approximately 115 kDa

Purity

Greater than 85% as determined by reducing SDS-PAGE

Endotoxin Level

<1 EU/μg, determined by LAL method.

Documentation

HK3/Hexokinase-3 Protein, Human (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
HK3/Hexokinase-3 Protein, Human (sf9, His-GST)
Cat. No.:
HY-P74881
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