HSP70/DnaK Protein, E. coli (P.pastoris, His)

HSP70/DnaK protein, a molecular chaperone, responds primarily to stress, notably heat shock. It plays a crucial role in aiding the proper folding of proteins during cellular stress, preventing misfolding or aggregation. The chaperone activity is vital for cellular homeostasis and survival under adverse conditions, where maintaining correct protein conformation becomes challenging. HSP70/DnaK's ability to recognize and assist in refolding misfolded or denatured proteins is essential for cellular resilience, preventing proteotoxicity induced by stressors, especially elevated temperatures. HSP70/DnaK Protein, E. coli (P.pastoris, His) is the recombinant E. coli-derived HSP70/DnaK protein, expressed by P. pastoris, with N-6*His labeled tag. The total length of HSP70/DnaK Protein, E. coli (P.pastoris, His) is 638 a.a., with molecular weight of ~71.1 kDa.

HSP70/DnaK protein functions as a molecular chaperone, primarily responding to stress conditions, such as heat shock. In times of cellular stress, this protein plays a crucial role in assisting the proper folding of other proteins, preventing misfolding or aggregation. The chaperone activity of HSP70/DnaK is especially vital for cellular homeostasis and survival under adverse environmental conditions, where maintaining the correct conformation of proteins becomes challenging. The protein's ability to recognize and assist in the refolding of misfolded or denatured proteins is essential for cellular resilience and the prevention of proteotoxicity induced by various stressors, particularly elevated temperatures.

E.coli

P. pastoris

N-6*His

Q1RGI8 (M1-K638)

58463508  [NCBI]

MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQDGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAWVEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRIAGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEVLATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSAQQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDIDDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLLDVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAADNKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLNEDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIESALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDDDVVDAEFEEVKDKK

Approximately 71.1 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against solution in 20 mM Tris-HC1, 0.5 M NaCl, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.