IA2 Protein, Human (365a.a, His)

The IA2 protein is critical in the vesicle-mediated secretion process and is essential for the accumulation of secretory vesicles in the hippocampus, pituitary gland, and pancreatic islets. IA2 is critical for insulin-related functions, ensuring normal levels of insulin-containing vesicles, preventing degradation and promoting glucose-stimulated insulin secretion. IA2 Protein, Human (365a.a, His) is the recombinant human-derived IA2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of IA2 Protein, Human (365a.a, His) is 375 a.a., with molecular weight of 45-50 kDa.

The IA2 protein plays a pivotal role in vesicle-mediated secretory processes, with essential functions extending across various tissues. It is required for the normal accumulation of secretory vesicles in key areas such as the hippocampus, pituitary, and pancreatic islets. Particularly crucial for insulin-related processes, IA2 is necessary for the accumulation of normal levels of insulin-containing vesicles, preventing their degradation and contributing to insulin secretion in response to glucose stimuli. IA2's influence also extends to neurotransmitter regulation in the brain, where it is required for the normal accumulation of norepinephrine, dopamine, and serotonin. Interestingly, in females, IA2 is uniquely indispensable for the normal accumulation and secretion of pituitary hormones like luteinizing hormone (LH) and follicle-stimulating hormone (FSH). Moreover, IA2 plays a role in maintaining normal levels of renin expression and release. Despite lacking intrinsic enzyme activity, IA2 may regulate catalytic active protein-tyrosine phosphatases, such as PTPRA, through dimerization. The intricate dynamics of IA2 are further highlighted in its interaction with ICA512-TMF, where it modulates the dynamics and exocytosis of insulin secretory granules, proposing a tethering mechanism to the actin cytoskeleton dependent on UTRN and regulated by ICA512-CFF dimerization.

Human

E. coli

N-6*His

Q16849-2 (R576-Q950)

5798  [NCBI]

RQQDKERLAALGPEGAHGDTTFEYQDLCRQHMATKSLFNRAEGPPEPSRVSSVSSQFSDAAQASPSSHSSTPSWCEEPAQANMDISTGHMILAYMEDHLRNRDRLAKEWQALCAYQAEPNTCATAQGEGNIKKNRHPDFLPYDHARIKLKVESSPSRSDYINASPIIEHDPRMPAYIATQGPLSHTIADFWQMVWESGCTVIVMLTPLVEDGVKQCDRYWPDEGASLYHVYEVNLVSEHIWCEDFLVRSFYLKNVQTQETRTLTQFHFLSWPAEGTPASTRPLLDFRRKVNKCYRGRSCPIIVHCSDGAGRTGTYILIDMVLNRMAKGVKEIDIAATLEHVRDQRPGLVRSKDQFEFALTAVAEEVNAILKALPQ

45-50 kDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 150 mM NaCl, pH 8.0 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.