IDI2 Protein, Human (C-His)

IDI2 protein plays a crucial role in catalyzing the 1,3-allylic rearrangement of the homoallylic substrate isopentenyl (IPP), leading to the formation of its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). This enzymatic activity is a key step in the biosynthetic pathway, contributing to the generation of essential building blocks for various cellular processes. IDI2 Protein, Human (C-His) is the recombinant human-derived IDI2 protein, expressed by E. coli , with C-8*His labeled tag. The total length of IDI2 Protein, Human (C-His) is 225 a.a., with molecular weight of ~27 kDa.

Isopentenyl-diphosphate Delta-isomerase 2 (IDI2) plays a pivotal role in isoprenoid biosynthesis by catalyzing the 1,3-allylic rearrangement of isopentenyl diphosphate (IPP), converting it to its highly electrophilic allylic isomer, dimethylallyl diphosphate (DMAPP). This enzymatic activity is a crucial step in the mevalonate pathway, which is responsible for the production of isoprenoids, essential compounds involved in various cellular processes, including protein prenylation and sterol biosynthesis. IDI2's ability to catalyze the isomerization of IPP to DMAPP is fundamental to the generation of diverse isoprenoid compounds that serve as precursors for a wide range of biomolecules in cellular metabolism.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

C-8*His

Q9BXS1 (D3-V227)

91734  [NCBI]

DINLDWVDRRQLQRLEEMLIVVDENDKVIGADTKRNCHLNENIEKGLLHRAFSVVLFNTKNRILIQQRSDTKVTFPGYFTDSCSSHPLYNPAELEEKDAIGVRRAAQRRLQAELGIPGEQISPEDIVFMTIYHHKAKSDRIWGEHEICYLLLVRKNVTLNPDPSETKSILYLSQEELWELLEREARGEVKVTPWLRTIAERFLYRWWPHLDDVTPFVELHKIHRV

Approximately 27 KDa

• 
  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.