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IKB beta/NFKBIB Protein, Human (His)

Cat. No.: HY-P76986
COA Handling Instructions

IKB beta/NFKBIB protein effectively inhibits NF-kappa-B and prevents NF-kappa-B activation by forming a cytoplasmic complex. After cell stimulation, the non-phosphorylated form is resynthesized, binds to NF-kappa-B, aids nuclear transport, and protects NF-kappa-B from NFKBIA-dependent inactivation. IKB beta/NFKBIB Protein, Human (His) is the recombinant human-derived IKB beta/NFKBIB protein, expressed by E. coli , with N-6*His labeled tag.

For research use only. We do not sell to patients.

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Description

IKB beta/NFKBIB protein effectively inhibits NF-kappa-B and prevents NF-kappa-B activation by forming a cytoplasmic complex. After cell stimulation, the non-phosphorylated form is resynthesized, binds to NF-kappa-B, aids nuclear transport, and protects NF-kappa-B from NFKBIA-dependent inactivation. IKB beta/NFKBIB Protein, Human (His) is the recombinant human-derived IKB beta/NFKBIB protein, expressed by E. coli , with N-6*His labeled tag.

Background

IKB beta/NFKBIB protein functions as a potent inhibitor of NF-kappa-B by forming a complex with it and sequestering it in the cytoplasm. Upon cell stimulation, the unphosphorylated form is resynthesized, enabling its binding to NF-kappa-B and facilitating its transport to the nucleus. This protects NF-kappa-B from further NFKBIA-dependent inactivation. Additionally, its association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevents phosphorylation, rendering it more resistant to degradation and explaining its slower turnover. IKB beta/NFKBIB protein exhibits interactions with THRB, RELA, and REL, contributing to its regulatory role. Moreover, it interacts with COMMD1 and inhibitor kappa B-interacting Ras-like NKIRAS1 and NKIRAS2, adding further complexity to its modulatory functions.

Species

Human

Source

E. coli

Tag

N-His

Accession

Q15653-1/NP_002494.2 (M1-V356)

Gene ID
Molecular Construction
N-term
6*His
NFKBIB (M1-V356)
Accession # Q15653-1/NP_002494.2
C-term
Synonyms
NF-kappa-B inhibitor beta; NF-kappa-BIB; TRIP-9; IKBB
AA Sequence

MAGVACLGKAADADEWCDSGLGSLGPDAAAPGGPGLGAELGPGLSWAPLVFGYVTEDGDTALHLAVIHQHEPFLDFLLGFSAGTEYMDLQNDLGQTALHLAAILGETSTVEKLYAAGAGLCVAERRGHTALHLACRVGAHACARALLQPRPRRPREAPDTYLAQGPDRTPDTNHTPVALYPDSDLEKEEEESEEDWKLQLEAENYEGHTPLHVAVIHKDVEMVRLLRDAGADLDKPEPTCGRSPLHLAVEAQAADVLELLLRAGANPAARMYGGRTPLGSAMLRPNPILARLLRAHGAPEPEGEDEKSGPCSSSSDSDSGDEGDEYDDIVVHSSRSQTRLPPTPASKPLPDDPRPV

Molecular Weight

Approximately 45 kDa.

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IKB beta/NFKBIB Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IKB beta/NFKBIB Protein, Human (His)
Cat. No.:
HY-P76986
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