IL-18 Protein, Rhesus Macaque (His)

IL-18 Protein, a pro-inflammatory cytokine, crucially participates in epithelial barrier repair and modulates immune responses, particularly in Th1 cell and NK cell immune responses. Binding to IL18R1 and IL18RAP receptors, IL-18 forms a signaling ternary complex activating NF-kappa-B, inducing inflammatory mediators. Synergizing with IL-12, it stimulates IFNG synthesis from Th1 cells and NK cells. Additionally, IL-18 is involved in transducing inflammation during pyroptosis, releasing its mature form through the gasdermin-D (GSDMD) pore. IL-18 Protein, Rhesus Macaque (His) is the recombinant Rhesus Macaque-derived IL-18 protein, expressed by E. coli, with N-6*His, labeled tag. The total length of IL-18 Protein, Rhesus Macaque (His) is 192 a.a., with molecular weight of ~24.39 kDa.

IL-18 Protein is a pro-inflammatory cytokine that plays a crucial role in epithelial barrier repair and the modulation of immune responses, specifically in polarized T-helper 1 (Th1) cell and natural killer (NK) cell immune responses. Upon binding to its receptors IL18R1 and IL18RAP, IL-18 forms a signaling ternary complex that activates NF-kappa-B, resulting in the synthesis of inflammatory mediators. It also works synergistically with IL-12/interleukin-12 to induce the synthesis of IFNG from Th1 cells and NK cells. Furthermore, IL-18 is involved in transducing inflammation downstream of pyroptosis, where its mature form is selectively released into the extracellular space through the gasdermin-D (GSDMD) pore.

Measured by its ability to induce IFN-gamma secretion by KG-1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED₅₀ for this effect is 1.095 ng/mL in the presence of 15 ng/mL TNF-alpha, corresponding to a specific activity is 9.13×1010⁵ U/mg.

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  Measured by its ability to induce IFN-gamma secretion by KG‑1 human acute myelogenous leukemia cells in the presence of TNF-alpha. The ED₅₀ for this effect is 1.095 ng/mL in the presence of 15 ng/mL TNF-alpha, corresponding to a specific activity is 9.13×10⁵ U/mg.

Rhesus Macaque

E. coli

N-6*His;

Q9BGI5/NP_001028006.1 (A2-D193)

574151  [NCBI]

AAEPAEDNCINFVAMKFIDSTLYFIAEDDENLESDYFGKLESKLSIIRNLNDQVLFIDQGNRPLFEDMTDSDCRDNAPRTIFIINMYKDSQPRGMAVAISVKCEKISTLSCENRIISFKEMNPPDNIKDTKSDIIFFQRSVPGHDNKMQFESSSYEGYFLACEKERDLYKLILKKKDELGDRSIMFTVQNED

Approximately 24.39 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.