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  5. IL-18BP Protein, Human (Biotinylated, HEK293, His-Avi)

IL-18BP Protein, Human (Biotinylated, HEK293, His-Avi)

Cat. No.: HY-P78156
SDS COA Handling Instructions

IL-18BP isoform A, a potent regulator, binds to IL-18, effectively inhibiting its activity. Serving as a suppressor, IL-18BP restrains early TH1 cytokine responses, playing a pivotal role in modulating immune reactions and maintaining cytokine balance. IL-18BP Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived IL-18BP protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of IL-18BP Protein, Human (Biotinylated, HEK293, His-Avi) is 164 a.a., with molecular weight of 42-62 kDa.

For research use only. We do not sell to patients.

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Size Price Stock Quantity
20 μg $195 In-stock
50 μg $430 In-stock
100 μg $725 Get quote
> 100 μg   Get quote  

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Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

IL-18BP isoform A, a potent regulator, binds to IL-18, effectively inhibiting its activity. Serving as a suppressor, IL-18BP restrains early TH1 cytokine responses, playing a pivotal role in modulating immune reactions and maintaining cytokine balance. IL-18BP Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived IL-18BP protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of IL-18BP Protein, Human (Biotinylated, HEK293, His-Avi) is 164 a.a., with molecular weight of 42-62 kDa.

Background

IL-18 binding protein (IL-18BP), specifically isoform A, serves as a potent regulator by binding to IL-18 and effectively inhibiting its activity. Acting as a suppressor, IL-18BP functions to restrain the early TH1 cytokine response, highlighting its pivotal role in modulating immune reactions and maintaining cytokine balance.

Biological Activity

Immobilized Human IL-18 at 2 μg/mL (100μL/Well) on the plate. Dose response curve for Biotinylated Human IL-18BP His with the EC50 of 6.1-21 ng/mL determined by ELISA.

Species

Human

Source

HEK293

Tag

C-Avi;C-His

Accession

O95998-2 (T31-G194)

Gene ID
Molecular Construction
N-term
IL-18BP (T31-G194)
Accession # O95998-2
His-Avi
C-term
Synonyms
IL-18BP; Igifbp
Molecular Weight

42-62 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

IL-18BP Protein, Human (Biotinylated, HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IL-18BP Protein, Human (Biotinylated, HEK293, His-Avi)
Cat. No.:
HY-P78156
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