IL-18BP Protein, Human (HEK293, His)

IL-18BP isoform A, a potent regulator, binds to IL-18, effectively inhibiting its activity. Serving as a suppressor, IL-18BP restrains early TH1 cytokine responses, playing a pivotal role in modulating immune reactions and maintaining cytokine balance. IL-18BP Protein, Human (HEK293, His) is the recombinant human-derived IL-18BP protein, expressed by HEK293 , with C-His labeled tag. The total length of IL-18BP Protein, Human (HEK293, His) is 164 a.a., with molecular weight of 40-65 kDa.

IL-18 binding protein (IL-18BP), specifically isoform A, serves as a potent regulator by binding to IL-18 and effectively inhibiting its activity. Acting as a suppressor, IL-18BP functions to restrain the early TH1 cytokine response, highlighting its pivotal role in modulating immune reactions and maintaining cytokine balance.

1.Immobilized Human IL-18, No Tag at 5 μg/mL (100 μl/well) on the plate. Dose response curve for Human IL-18BP, His Tag with the EC₅₀ of ≤15 ng/mL determined by ELISA.
2.Human IL-18BP, His Tag captured on CM5 Chip via Anti-his antibody can bind Human IL-18, No Tag with an affinity constant of 0.3-0.5 nM as determined in SPR assay (Biacore T200).
3.Measured by its ability to inhibit the IL-18-induced response of human natural killer lymphoma NK-92 cells. The ED₅₀ for this effect is 0.04 - 0.08 µg/mL in the presence of 30 ng/mL of recombinant Human IL-18.

Human

HEK293

C-His

O95998-2 (T31-G194)

10068  [NCBI]

40-65 kDa

Greater than 95% as determined by Tris-Bis PAGE.

Lyophilized powder

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.