2. Recombinant Proteins
  3. Cytokines and Growth Factors
  4. Interleukin & Receptors Neurotrophic Factors
  5. IL-6
  6. IL-6 Protein, Rabbit (P.pastoris, His)

IL-6 Protein, Rabbit (P.pastoris, His)

Cat. No.: HY-P702586
COA Handling Instructions

IL-6 Protein, Rabbit (P.pastoris, His) is the recombinant rabbit-derived IL-6, expressed by P. pastoris , with N-6*His labeled tag. ,

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

Size Price Stock Quantity
20 μg $210 In-stock
50 μg $450 In-stock
100 μg $720 In-stock
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Top Publications Citing Use of Products

  • Biological Activity

  • Technical Parameters

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Description

IL-6 Protein, Rabbit (P.pastoris, His) is the recombinant rabbit-derived IL-6, expressed by P. pastoris , with N-6*His labeled tag. ,
Species

Rabbit
Source

P. pastoris
Tag

N-6*His
Accession

Q9MZR1 (F27-T241)
Gene ID

100008733
Synonyms
Interleukin-6
AA Sequence

FPTSAPVREDSNTKASPDKTLTPPGRTIESIRSILETIKELRKEMCDHDVNCMNRKEALAEVNLHLPRLIEEDGCFPPAVNNETCLLRITSGLMEFRMYLEHLQAKFRSDEENTRVSMVLKNIQHLIKTLRPKVKNLNEEATLKPAVAVSLMENLQQKNQWLKTTTIHFILRGLTNFLEFTLRAVDLMECGCPCLRNFMGSASHGQNTPSCPLDT

Molecular Weight

Apparently 33 kDa
Purity

Greater than 90 % as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.
Formulation

Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6%Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Shipping

Room temperature in continental US; may vary elsewhere.
Documentation

COA (220 KB)

Handling Instructions (2659 KB)

IL-6 Protein, Rabbit (P.pastoris, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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