Importin alpha-1/KPNA2 Protein, Human (His)

Importin alpha-1/KPNA2 is essential for nuclear protein import, acting as an adapter for the nuclear receptor KPNB1. It directly binds the substrate to the nuclear localization signal to form a complex that docks with the nuclear pore complex mediated by KPNB1. Importin alpha-1/KPNA2 Protein, Human (His) is the recombinant human-derived Importin alpha-1/KPNA2 protein, expressed by E. coli , with N-6*His labeled tag. The total length of Importin alpha-1/KPNA2 Protein, Human (His) is 529 a.a., with molecular weight of ~60.0 kDa.

Importin alpha-1/KPNA2 plays a pivotal role in nuclear protein import by serving as an adapter protein for the nuclear receptor KPNB1. It exhibits specific and direct binding to substrates containing either a simple or bipartite nuclear localization signal (NLS) motif. The importin/substrate complex docks to the nuclear pore complex (NPC) through the mediation of KPNB1, binding to nucleoporin FxFG repeats. Subsequently, the complex is translocated through the pore via an energy-requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta, leading to the separation of the three components. Importin-alpha and -beta are then re-exported from the nucleus to the cytoplasm, where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is believed to be determined by the asymmetric distribution of GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Importin alpha-1 forms a heterodimer with KPNB1 and interacts with various proteins, including ANP32E, CSE1L, RAN, PLAG1, APEX1, FRG1, ARL4A, CTNNBL1, NBN, SNAI1, SNAI2, BAG6, AIFM2, and RSL1D1.

Human

E. coli

N-6*His

P52292 (M1-F529)

3838  [NCBI]

MSTNENANTPAARLHRFKNKGKDSTEMRRRRIEVNVELRKAKKDDQMLKRRNVSSFPDDATSPLQENRNNQGTVNWSVDDIVKGINSSNVENQLQATQAARKLLSREKQPPIDNIIRAGLIPKFVSFLGRTDCSPIQFESAWALTNIASGTSEQTKAVVDGGAIPAFISLLASPHAHISEQAVWALGNIAGDGSVFRDLVIKYGAVDPLLALLAVPDMSSLACGYLRNLTWTLSNLCRNKNPAPPIDAVEQILPTLVRLLHHDDPEVLADTCWAISYLTDGPNERIGMVVKTGVVPQLVKLLGASELPIVTPALRAIGNIVTGTDEQTQVVIDAGALAVFPSLLTNPKTNIQKEATWTMSNITAGRQDQIQQVVNHGLVPFLVSVLSKADFKTQKEAVWAVTNYTSGGTVEQIVYLVHCGIIEPLMNLLTAKDTKIILVILDAISNIFQAAEKLGETEKLSIMIEECGGLDKIEALQNHENESVYKASLSLIEKYFSVEEEEDQNVVPETTSEGYTFQVQDGAPGTFNF

Approximately 60.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 1 mM DTT, 20% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.