1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Protein Tyrosine Kinases
  4. IL-2 Inducible T-Cell Kinase (ITK/TSK)
  5. ITK Protein, Mouse (sf9, His-GST)

ITK Protein, Mouse (sf9, His-GST)

Cat. No.: HY-P74795
SDS COA Handling Instructions

ITK protein is a tyrosine kinase that plays a crucial role in directing adaptive immune responses by controlling the development, function, and differentiation of conventional T cells and unconventional NKT cells.After T cell receptor (TCR) activation by antigen-presenting cells (APC), ITK is recruited to the cell membrane and phosphorylated by LCK, thereby making it fully activated.ITK Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived ITK protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

For research use only. We do not sell to patients.

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50 μg $350 In-stock
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  • Biological Activity

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Description

ITK protein is a tyrosine kinase that plays a crucial role in directing adaptive immune responses by controlling the development, function, and differentiation of conventional T cells and unconventional NKT cells.After T cell receptor (TCR) activation by antigen-presenting cells (APC), ITK is recruited to the cell membrane and phosphorylated by LCK, thereby making it fully activated.ITK Protein, Mouse (sf9, His-GST) is the recombinant mouse-derived ITK protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.

Background

ITK protein, a tyrosine kinase, assumes a critical role in orchestrating the adaptive immune response by governing the development, function, and differentiation of both conventional T-cells and nonconventional NKT-cells. Upon activation of the T-cell receptor (TCR) by antigen-presenting cells (APC), ITK is recruited to the cell membrane in close proximity to the stimulated TCR receptor, where it undergoes phosphorylation by LCK. This phosphorylation initiates ITK autophosphorylation, culminating in its full activation. Once activated, ITK phosphorylates PLCG1, activating this lipase and triggering the subsequent cleavage of its substrates. Consequently, calcium is released from the endoplasmic reticulum into the cytoplasm, facilitating the translocation of the nuclear activator of activated T-cells (NFAT) into the nucleus to execute its transcriptional functions. ITK also phosphorylates two essential adapter proteins, the linker for activation of T-cells/LAT protein and LCP2, leading to the recruitment of various signaling molecules, such as VAV1. This intricate signaling cascade ultimately results in lymphokine production, T-cell proliferation, and differentiation. Moreover, ITK is indispensable for TCR-mediated calcium response in gamma-delta T-cells, potentially influencing the transcriptomic signature in the Vgamma2-positive subset of immature gamma-delta T-cells. Additionally, ITK phosphorylates TBX21 at 'Tyr-525,' mediating its interaction with GATA3. The multifaceted functions of ITK underscore its pivotal role in shaping the adaptive immune response.

Biological Activity

The specific activity of ITK was determined to be 54.33 nmol/min/mg using a myelin basic protein (MBP) substrate.

Species

Mouse

Source

Sf9 insect cells

Tag

N-His;N-GST

Accession

Q03526 (R351-L619)

Gene ID
Molecular Construction
N-term
His-GST
ITK (R351-L619)
Accession # Q03526
C-term
Synonyms
Tyrosine-protein kinase ITK/TSK; IL-2-inducible T-cell kinase; ITK; EMT; LYK
Molecular Weight

Approximately 58 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, 100 mM NaCl, pH 7.5, 10% Glycerol, 0.5 mM GSH. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

ITK Protein, Mouse (sf9, His-GST) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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ITK Protein, Mouse (sf9, His-GST)
Cat. No.:
HY-P74795
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