Kallikrein-8 Protein, Human (HEK293, His)

Kallikrein-8 protein is a serine protease that degrades proteins such as casein, fibrinogen, kininogen, fibronectin, and type IV collagen. It cleaves L1CAM in response to neural activity, promoting neurite growth in hippocampal neurons. Kallikrein-8 Protein, Human (HEK293, His) is the recombinant human-derived Kallikrein-8 protein, expressed by HEK293 , with C-His labeled tag.

Kallikrein-8 Protein is a serine protease known for its ability to degrade various proteins including casein, fibrinogen, kininogen, fibronectin, and collagen type IV. It also cleaves L1CAM in response to heightened neural activity, leading to neurite outgrowth and fasciculation in cultured hippocampal neurons. This protein plays a crucial role in the formation and maturation of orphan and small synaptic boutons in the Schaffer-collateral pathway, thereby regulating Schaffer-collateral long-term potentiation in the hippocampus and contributing to memory acquisition and synaptic plasticity. Additionally, Kallikrein-8 is involved in skin desquamation, keratinocyte proliferation, and plays a role in the secondary phase of pathogenesis following spinal cord injury.

Measured by its ability to cleave the fluorogenic peptide substrate BocVPRAMC. The specific activity is > 400pmol/min/μg

Human

HEK293

C-His

O60259-1 (Q29-G260)

11202  [NCBI]

Approximately 36 kDa

Greater than 95% as determined by reducing SDS-PAGE

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.