KIRREL3/NEPH2 Protein, Mouse (HEK293, His)

The KIRREL3/NEPH2 protein plays a critical role in the formation of precise synapses, particularly in hippocampal mossy fiber synapses, where it is responsible for coordinating mossy fiber filopodia. It acts in a homologous manner to stabilize filopodia contacts and facilitate subsequent synapse formation. KIRREL3/NEPH2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived KIRREL3/NEPH2 protein, expressed by HEK293 , with C-His labeled tag. The total length of KIRREL3/NEPH2 Protein, Mouse (HEK293, His) is 514 a.a., with molecular weight of ~60-80 kDa.

The KIRREL3/NEPH2 protein emerges as a pivotal synaptic adhesion molecule essential for the precise formation of target-specific synapses, particularly notable at hippocampal mossy fiber synapses. Its significance extends to the orchestration of mossy fiber filopodia, critical synaptic structures linking dentate granule and GABA neurons. Functionally, KIRREL3/NEPH2 likely serves as a homophilic adhesion molecule, fostering trans-cellular interactions and playing a key role in stabilizing mossy fiber filipodia contacts, thereby facilitating subsequent synapse formation. Beyond the hippocampus, KIRREL3/NEPH2 is vital for the coalescence of vomeronasal sensory neuron axons. Moreover, it may play a role in the hematopoietic supportive capacity of stroma cells, with its secreted extracellular domain directly supporting hematopoietic stem cells. Functioning as a homodimer, KIRREL3/NEPH2 mediates homophilic interactions crucial for promoting cell adhesion. Additionally, it engages in a spectrum of protein-protein interactions, including the formation of heterodimers with NPHS1, interactions with NPHS2/podocin, CASK, MAP1B, MYO16, ATP1B1, SHMT2, and UFC1, underscoring its multifaceted and integral role in diverse cellular processes.

Mouse

HEK293

C-His

Q8BR86 (L22-A535)

67703  [NCBI]

LQKRGCCLVLGYMAKDKFRRMNEGQVYSFSQQPQDQVVVSGQPVTLLCAIPEYDGFVLWIKDGLALGVGRDLSSYPQYLVVGNHLSGEHHLKILRAELQDDAVYECQAIQAAIRSRPARLTVLVPPDDPIILGGPVISLRAGDPLNLTCHADNAKPAASIIWLRKGEVINGATYSKTLLRDGKRESIVSTLFISPGDVENGQSIVCRATNKAIPGGKETSVTIDIQHPPLVNLSVEPQPVLEDNIVTFHCSAKANPAVTQYRWAKRGHIIKEASGELYRTTVDYTYFSEPVSCEVTNALGSTNLSRTVDVYFGPRMTSEPQSLLVDLGSDAVFSCAWIGNPSLTIVWMKRGSGVVLSNEKTLTLKSVRQEDAGKYVCRAVVPRVGAGEREVTLTVNGPPIISSTQTQHALHGEKGQIKCFIRSTPPPDRIAWSWKENVLESGTSGRYTVETVNTEEGVISTLTISNIVRADFQTIYNCTAWNSFGSDTEIIRLKEQGSEMKSGAGLEAESVPMA

Approximately 60-80 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.