Ku70-Ku80 Heterodimer Protein, Human (sf9, His)

The Ku70, a single-stranded DNA-dependent ATP-dependent helicase, is pivotal in DNA non-homologous end joining (NHEJ), central to double-strand break repair and V(D)J recombination. Operating as a DNA helicase II complex with a cell cycle-dependent preference for double-stranded DNA fork-like ends in the 3'-5' direction, it recognizes and binds to broken DNA ends during NHEJ, protecting them from resection. As a DNA-PK regulatory subunit, it enhances PRKDC's affinity to DNA, stabilizes broken DNA ends, and facilitates ligation in NHEJ. The heterodimer may act as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase) near double-strand breaks. Beyond DNA repair, it regulates transcription, osteocalcin expression, early ribosome assembly steps, and forms complexes involved in the innate immune response, showcasing its multifaceted cellular functions. Ku70-Ku80 Heterodimer Protein, Human (sf9, His) is a recombinant protein dimer complex containing human-derived Ku70-Ku80 Heterodimer protein, expressed by Sf9 insect cells, with N-His labeled tag. Ku70-Ku80 Heterodimer Protein, Human (sf9, His), has molecular weight of ~70 & 85 kDa, respectively.

The Ku70 is a single-stranded DNA-dependent ATP-dependent helicase crucial for DNA non-homologous end joining (NHEJ), playing a central role in double-strand break repair and V(D)J recombination. Functioning as a DNA helicase II complex, it exhibits a cell cycle-dependent preference for fork-like ends of double-stranded DNA and operates in the 3'-5' direction. During NHEJ, the heterodimer recognizes and binds to broken DNA ends, protecting them from further resection. It acts as a regulatory subunit of the DNA-dependent protein kinase complex (DNA-PK), significantly enhancing the catalytic subunit PRKDC's affinity to DNA. The Ku70 is implicated in stabilizing broken DNA ends and bringing them together for the subsequent ligation step in NHEJ. Additionally, it may serve as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), facilitating the elimination of 5' deoxyribose-5-phosphate at abasic sites near double-strand breaks. Beyond its role in DNA repair, the heterodimer participates in the regulation of transcription, osteocalcin expression, and the early steps of ribosome assembly. It also forms complexes involved in the innate immune response and associates with various interacting partners, highlighting its multifaceted functions in cellular processes.

Human

Sf9 insect cells

N-His

P13010 (M1-I732)&P12956 (M1-D609)

7520  [NCBI]&2547  [NCBI]

Approximately 70&85 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.